Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha2-gliadin 33-mer peptide | POP inhibitor | Sus scrofa | |
JTP-4819 | - |
Sus scrofa | |
additional information | specific dipeptidyl peptidase IV inhibitor HIV-1 tat (1-9) fragment with sequence H-Met-Asp-Pro-Val-Asp-Pro-Asn-Ile-Glu-OH | Sus scrofa | |
N-carbobenzoxy-Leu-Leu-Leu-COH | the proteasome inhibitor | Sus scrofa | |
N-carbobenzoxy-prolyl-prolinal | ZPP | Sus scrofa | |
phenylmethanesulfonyl fluoride | PE and POP are partially resistant to phenylmethanesulfonyl fluoride, a generic serine protease inhibitor that has a low efficiency in inhibiting POP | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sus scrofa | 5737 | - |
extracellular | - |
Sus scrofa | - |
- |
membrane | - |
Sus scrofa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sus scrofa | |
Mn2+ | - |
Sus scrofa | |
additional information | no significant differences between membrane PE and POP regarding the sensitivity to the metals | Sus scrofa | |
NaCl | - |
Sus scrofa | |
Ni2+ | - |
Sus scrofa | |
Zn2+ | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
higher salt concentrations used, membrane PE activity elutes with a molecular mass of 65 kDa | Sus scrofa |
80000 | - |
theoretical | Sus scrofa |
95000 | - |
membrane PE, conditions: 20 mm potassium phosphate, gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00017 | - |
high-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
0.0002 | - |
washed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.00045 | - |
medium-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
0.0005 | - |
total membranes, purification of mPOP from a total membrane preparation | Sus scrofa |
0.0005 | - |
washed membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
0.00061 | - |
low-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
0.001 | - |
crude extract, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.0014 | - |
unwashed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.0033 | - |
Triton X-100 extraction, purification of mPOP from a total membrane preparation | Sus scrofa |
0.0048 | - |
4 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.0074 | - |
DEAE, purification of mPOP from a total membrane preparation | Sus scrofa |
0.009 | - |
0.5 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.0138 | - |
water wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
0.039 | - |
phenyl-sepharose, purification of mPOP from a total membrane preparation | Sus scrofa |
0.169 | - |
hydroxylapatite, purification of mPOP from a total membrane preparation | Sus scrofa |
0.857 | - |
second DEAE, purification of mPOP from a total membrane preparation | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-CDPGYIGSR-NH2 + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
H-MDPVDPNIE-OH + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
H2N-QLQPFPQPQLPY-OH + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
additional information | substance P, arginine-vasopressin, thyroliberin and gonadoliberin are proposed physiological substrates of this protease. No ZPP-sensitive cleavage occurred with the 33-mer, PEP-3, PEP-26, PEP-48_2, and PEP-50 | Sus scrofa | ? | - |
? | |
N-carbobenzoxy-Gly-Pro-7-amido-4-methyl-coumarin + H2O | - |
Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane-bound PE | - |
Sus scrofa |
mPOP | - |
Sus scrofa |
POP | - |
Sus scrofa |
prolyl endopeptidase | - |
Sus scrofa |