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Literature summary for 3.4.21.117 extracted from
- Substrate specificity of two kallikrein family gene products isolated from the rat submandibular gland (1990), FEBS Lett., 265, 137-140.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Soybean trypsin inhibitor | strong inhibition | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9000 | - |
x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
| 19000 | - |
x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cleavage of proteins with aromatic side chains in the P1 position | enzyme exhibits a marked preference for cleavage downstream of Arg residues | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submandibular gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| additional information | no kininogenase activity | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme exhibits a marked preference for cleavage downstream of Arg residues | Rattus norvegicus | ? | - |
? | |
| o-aminobenzoyl-FRAPR-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | ? | - |
? | |
| o-aminobenzoyl-FRLVR-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | o-aminobenzoyl-FR + LVR-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
| o-aminobenzoyl-SVIRRVQ-(2,4-dinitrophenyl)ethylenediamine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| o-aminobenzoyl-TSVIRRPQ-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | ? | - |
? | |
| PFR-7-amido-4-methylcoumarin + H2O | best substrate | Rattus norvegicus | ? | - |
? | |
| polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
| tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Leu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
| tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tissue kallikrein | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Rattus norvegicus |
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