BRENDA - Enzyme Database
show all sequences of 3.4.21.101

Sedolisin-B

Oda, K.; Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press 2, 1888-1889 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Xanthomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
isovaleryl-tyrosyl-leucyl-tyrosinal
tyrostatin
Xanthomonas sp.
additional information
no inhibition by pepstatin, 2,3-diamino naphthalene and 1,2-Epoxy-3-(p-nitrophenoxy)propane
Xanthomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0036
-
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37C
Xanthomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
2 * 41000, SDS-PAGE
Xanthomonas sp.
84000
-
recombinant protein from Escherichia coli
Xanthomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Xanthomonas sp.
Q60106
T-22
-
Xanthomonas sp. T-22
Q60106
T-22
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Acid-denatured hemoglobin + H2O
-
665088
Xanthomonas sp.
?
-
-
-
?
Acid-denatured hemoglobin + H2O
-
665088
Xanthomonas sp. T-22
?
-
-
-
?
casein + H2O
-
665088
Xanthomonas sp.
?
-
-
-
?
casein + H2O
-
665088
Xanthomonas sp. T-22
?
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
pH 3.5, 37C
665088
Xanthomonas sp.
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
pH 3.5, 37C
665088
Xanthomonas sp. T-22
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 41000, SDS-PAGE
Xanthomonas sp.
Synonyms
Synonyms
Commentary
Organism
sedolisin-B
-
Xanthomonas sp.
Xanthomonas serine-carboxyl peptidase
-
Xanthomonas sp.
XSCO
-
Xanthomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
52.2
-
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37C
Xanthomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7
-
for casein digestion
Xanthomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000021
-
isovaleryl-tyrosyl-leucyl-tyrosinal
-
Xanthomonas sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Xanthomonas sp.
-
-
4
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Xanthomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
isovaleryl-tyrosyl-leucyl-tyrosinal
tyrostatin
Xanthomonas sp.
additional information
no inhibition by pepstatin, 2,3-diamino naphthalene and 1,2-Epoxy-3-(p-nitrophenoxy)propane
Xanthomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000021
-
isovaleryl-tyrosyl-leucyl-tyrosinal
-
Xanthomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0036
-
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37C
Xanthomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
2 * 41000, SDS-PAGE
Xanthomonas sp.
84000
-
recombinant protein from Escherichia coli
Xanthomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Acid-denatured hemoglobin + H2O
-
665088
Xanthomonas sp.
?
-
-
-
?
Acid-denatured hemoglobin + H2O
-
665088
Xanthomonas sp. T-22
?
-
-
-
?
casein + H2O
-
665088
Xanthomonas sp.
?
-
-
-
?
casein + H2O
-
665088
Xanthomonas sp. T-22
?
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
pH 3.5, 37C
665088
Xanthomonas sp.
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
pH 3.5, 37C
665088
Xanthomonas sp. T-22
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 41000, SDS-PAGE
Xanthomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
52.2
-
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37C
Xanthomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7
-
for casein digestion
Xanthomonas sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Xanthomonas sp.
-
-
4
Other publictions for EC 3.4.21.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665088
Oda
-
Sedolisin-B ...
Xanthomonas sp., Xanthomonas sp. T-22
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
-
-
1
-
-
-
2
1
-
-
2
-
-
2
-
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-
-
-
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6
1
3
-
-
-
1
1
-
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-
1
1
-
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-
1
-
-
-
-
-
2
1
1
-
-
2
-
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-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
649090
Wlodawer
Structural and enzymatic prope ...
Xanthomonas sp.
Acta Biochim. Pol.
50
81-102
2003
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-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
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-
-
-
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4
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
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-
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651761
Narutaki
Subsite preferences of pepstat ...
Xanthomonas sp., Xanthomonas sp. T-22
J. Biochem.
125
75-81
1999
-
-
-
-
-
-
1
45
-
-
-
-
-
2
-
-
-
-
-
-
-
-
51
-
1
-
-
-
59
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
45
-
-
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-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
651950
Oyama
Identification of catalytic re ...
Xanthomonas sp.
J. Biol. Chem.
274
27815-27822
1999
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-
1
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4
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3
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-
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1
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1
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-
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2
-
1
-
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5
-
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-
1
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4
-
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3
-
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1
-
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-
2
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5
-
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-
-
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-
-
-
-
-
137236
Oda
Cloning, nucleotide sequence, ...
Pseudomonas sp., Pseudomonas sp. No. 101
J. Biol. Chem.
269
26518-26524
1994
-
-
1
-
-
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
-
1
-
-
-
2
-
-
-
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-
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1
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4
-
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1
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-
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4
-
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2
-
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-
-
-
-
-
-
-
-
137232
Oda
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. No. 101
Biochim. Biophys. Acta
923
463-469
1987
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-
-
-
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5
-
-
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2
-
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5
-
1
1
-
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-
1
-
4
1
-
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
-
1
1
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
137235
Oda
-
Purification and properties of ...
Xanthomonas sp.
Agric. Biol. Chem.
51
3073-3080
1987
-
-
-
-
-
-
4
-
-
1
2
-
-
1
-
1
1
-
-
1
1
-
2
1
-
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-