BRENDA - Enzyme Database
show all sequences of 3.4.21.101

Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria

Narutaki, S.; Dunn, B.M.; Oda, K.; J. Biochem. 125, 75-81 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by pepstatin. The hydrophilic nature of the S2' subsite appears to be a distinguishing feature of pepstatin-insensitive carboxyl proteinases
Xanthomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00214
-
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00224
-
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0026
-
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0039
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00445
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00451
-
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.005
-
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00523
-
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00531
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00532
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
pH 3.5, 37°C
Xanthomonas sp.
0.00605
-
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0063
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
pH 3.5, 37°C
Xanthomonas sp.
0.0071
-
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00716
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00746
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
pH 3.5, 37°C
Xanthomonas sp.
0.00763
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
pH 3.5, 37°C
Xanthomonas sp.
0.00782
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00824
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
pH 3.5, 37°C
Xanthomonas sp.
0.00932
-
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00944
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
pH 3.5, 37°C
Xanthomonas sp.
0.00977
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00982
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
0.00995
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
pH 3.5, 37°C
Xanthomonas sp.
0.00995
-
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0101
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0105
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0106
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0114
-
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0115
-
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.012
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0126
-
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.013
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0135
-
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0138
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0139
-
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0158
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0164
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0173
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0174
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0183
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0183
-
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0213
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0217
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0283
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0662
-
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthomonas sp.
-
T-22
-
Xanthomonas sp. T-22
-
T-22
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Phe-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Tyr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
additional information
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position
651761
Xanthomonas sp.
?
-
651761
Xanthomonas sp.
?
additional information
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position
651761
Xanthomonas sp. T-22
?
-
651761
Xanthomonas sp. T-22
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.037
0.23
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.04
1.97
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.8
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.97
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.1
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.4
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.6
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
2.2
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
2.6
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
3.4
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
6
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
8.45
-
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
8.57
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
15.3
-
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
15.5
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
pH 3.5, 37°C
Xanthomonas sp.
15.9
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.1
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.6
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.8
-
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.9
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
18.1
-
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
18.6
-
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
19.4
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
20
50
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
23.1
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
24.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
pH 3.5, 37°C
Xanthomonas sp.
26.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
pH 3.5, 37°C
Xanthomonas sp.
26.9
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
27.3
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
pH 3.5, 37°C
Xanthomonas sp.
27.6
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
pH 3.5, 37°C
Xanthomonas sp.
28.1
-
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
30.5
-
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
30.9
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
pH 3.5, 37°C
Xanthomonas sp.
31
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
pH 3.5, 37°C
Xanthomonas sp.
32.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
pH 3.5, 37°C
Xanthomonas sp.
34
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
35.7
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
36.7
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
37
-
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
38.3
-
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
39.8
-
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
40.4
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
pH 3.5, 37°C
Xanthomonas sp.
43.3
-
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
43.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
pH 3.5, 37°C
Xanthomonas sp.
48.8
-
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
56.8
-
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
57.3
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
57.9
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
60.5
-
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
62.4
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
71.3
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
73.9
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
79.4
-
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
87.9
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
96.4
-
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
101
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
pH 3.5, 37°C
Xanthomonas sp.
102
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
105
-
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
189
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
pH 3.5, 37°C
Xanthomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by pepstatin. The hydrophilic nature of the S2' subsite appears to be a distinguishing feature of pepstatin-insensitive carboxyl proteinases
Xanthomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00214
-
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00224
-
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0026
-
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0039
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00445
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00451
-
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.005
-
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00523
-
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00531
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00532
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
pH 3.5, 37°C
Xanthomonas sp.
0.00605
-
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0063
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
pH 3.5, 37°C
Xanthomonas sp.
0.0071
-
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00716
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00746
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
pH 3.5, 37°C
Xanthomonas sp.
0.00763
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
pH 3.5, 37°C
Xanthomonas sp.
0.00782
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00824
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
pH 3.5, 37°C
Xanthomonas sp.
0.00932
-
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00944
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
pH 3.5, 37°C
Xanthomonas sp.
0.00977
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.00982
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
0.00995
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
pH 3.5, 37°C
Xanthomonas sp.
0.00995
-
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0101
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0105
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0106
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0114
-
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0115
-
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.012
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0126
-
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.013
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0135
-
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0138
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0139
-
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0158
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0164
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0173
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0174
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0183
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0183
-
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0213
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0217
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0283
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.0662
-
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Phe-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp. T-22
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp. T-22
?
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Tyr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651761
Xanthomonas sp.
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
651761
Xanthomonas sp.
?
additional information
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position
651761
Xanthomonas sp.
?
-
651761
Xanthomonas sp.
?
additional information
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position
651761
Xanthomonas sp. T-22
?
-
651761
Xanthomonas sp. T-22
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.037
0.23
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.04
1.97
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.8
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
0.97
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.1
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.4
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
pH 3.5, 37°C
Xanthomonas sp.
1.6
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
2.2
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
2.6
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
3.4
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
6
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
8.45
-
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
8.57
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
pH 3.5, 37°C
Xanthomonas sp.
15.3
-
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
15.5
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
pH 3.5, 37°C
Xanthomonas sp.
15.9
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.1
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.6
-
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.8
-
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
16.9
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
18.1
-
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
18.6
-
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
19.4
-
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
20
50
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
23.1
-
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
24.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
pH 3.5, 37°C
Xanthomonas sp.
26.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
pH 3.5, 37°C
Xanthomonas sp.
26.9
-
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
27.3
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
pH 3.5, 37°C
Xanthomonas sp.
27.6
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
pH 3.5, 37°C
Xanthomonas sp.
28.1
-
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
30.5
-
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
30.9
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
pH 3.5, 37°C
Xanthomonas sp.
31
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
pH 3.5, 37°C
Xanthomonas sp.
32.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
pH 3.5, 37°C
Xanthomonas sp.
34
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
35.7
-
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
36.7
-
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
37
-
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
38.3
-
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
39.8
-
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
40.4
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
pH 3.5, 37°C
Xanthomonas sp.
43.3
-
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
43.7
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
pH 3.5, 37°C
Xanthomonas sp.
48.8
-
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
56.8
-
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
57.3
-
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
57.9
-
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
60.5
-
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
62.4
-
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
71.3
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
pH 3.5, 37°C
Xanthomonas sp.
73.9
-
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
79.4
-
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
87.9
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
pH 3.5, 37°C
Xanthomonas sp.
96.4
-
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
101
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
pH 3.5, 37°C
Xanthomonas sp.
102
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
105
-
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C
Xanthomonas sp.
189
-
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
pH 3.5, 37°C
Xanthomonas sp.
Other publictions for EC 3.4.21.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665088
Oda
-
Sedolisin-B ...
Xanthomonas sp., Xanthomonas sp. T-22
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
-
-
1
-
-
-
2
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
2
1
1
-
-
2
-
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
649090
Wlodawer
Structural and enzymatic prope ...
Xanthomonas sp.
Acta Biochim. Pol.
50
81-102
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651761
Narutaki
Subsite preferences of pepstat ...
Xanthomonas sp., Xanthomonas sp. T-22
J. Biochem.
125
75-81
1999
-
-
-
-
-
-
1
45
-
-
-
-
-
2
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
45
-
-
-
-
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
651950
Oyama
Identification of catalytic re ...
Xanthomonas sp.
J. Biol. Chem.
274
27815-27822
1999
-
-
1
-
4
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
137236
Oda
Cloning, nucleotide sequence, ...
Pseudomonas sp., Pseudomonas sp. No. 101
J. Biol. Chem.
269
26518-26524
1994
-
-
1
-
-
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
137232
Oda
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. No. 101
Biochim. Biophys. Acta
923
463-469
1987
-
-
-
-
-
-
5
-
-
-
2
-
-
5
-
1
1
-
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
-
1
1
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
137235
Oda
-
Purification and properties of ...
Xanthomonas sp.
Agric. Biol. Chem.
51
3073-3080
1987
-
-
-
-
-
-
4
-
-
1
2
-
-
1
-
1
1
-
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-