General Stability | Organism |
---|---|
addition of 1% bovine serum albumin to the purified enzyme improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C | Bos taurus |
enzyme solubilized by sodium deoxycholate and Triton X-100, -20°C or 4°C, 24 h, more than 50% loss of activity | Bos taurus |
trypsin solubilized enzyme, no loss of activity after 14 days at 4°C, 20% loss of activity after 14 days at -20°C | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Bos taurus | |
1,7-phenanthroline | - |
Bos taurus | |
4,7-phenanthroline | - |
Bos taurus | |
4-(2-aminoethyl)-benzenesulfonylfluoride | - |
Bos taurus | |
8-hydroxyquinoline | - |
Bos taurus | |
Cd2+ | - |
Bacillus amyloliquefaciens | |
Cd2+ | CdSO4 | Bos taurus | |
Dithiobisnitrobenzoate | - |
Bos taurus | |
dithiothreitol | - |
Bos taurus | |
EGTA | - |
Bos taurus | |
Hg2+ | - |
Bacillus amyloliquefaciens | |
Hg2+ | HgSO4 | Bos taurus | |
luteinizing hormone releasing hormone 1-5 | - |
Bos taurus | |
thioglycolic acid | - |
Bos taurus | |
Zn2+ | ZnSO4 | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00253 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus | |
0.4198 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoSO4 | activates | Bos taurus | |
additional information | metallopeptidase | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
214000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0208 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C or 4°C, 24 h, more than 50% loss of activity of the enzyme solubilized by sodium deoxycholate and Triton X-100 | Bos taurus |
4°C, 72 h, 10% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
4°C, 72 h, 60% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-pyroglutamyl-His-Pro-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | L-pyroglutamate + His-Pro-4-methylcoumarin 7-amide | - |
? | |
pyroglutamyl-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | pyroglutamate + 7-amino-4-methylcoumarin | - |
? | |
pyroglutamyl-His-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Gly | - |
? | |
pyroglutamyl-His-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Gly-NH2 | - |
? | |
pyroglutamyl-His-Pro-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly | - |
? | |
pyroglutamyl-His-Pro-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly-NH2 | - |
? | |
pyroglutamyl-Phe-Pro-NH2 + H2O | - |
Bos taurus | pyroglutamate + Phe-Pro-NH2 | - |
? | |
thyroliberin + H2O | - |
Bos taurus | pyroglutamate + His-Pro-NH2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable for at least 1 h | Bos taurus |
55 | - |
partial inactivation | Bos taurus |
60 | - |
2 min, total inactivation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.6 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 25% of maximal activity | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9.2 | rapid inactivation below pH 4.0 and above pH 9.2 | Bos taurus |