Application | Comment | Organism |
---|---|---|
medicine | membrane-associated farnesylated UCH-L1 is a therapeutic target for Parkinson's disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
FLAG-tagged UCH-L1 overexpression in the SH-SY5Y human neuroblastoma cell line, which endogenously expresses UCH-L1 and alpha-synuclein, can be cytotoxic at high expression levels in these cells. Coexpression of wild-type UCH-L1, but not of the protective S18Y variant, in COS-7 cells increases levels of transfected alpha-synuclein by inhibiting its degradation, the amount of UCH-L1M in transfected cells correlates with the intracellular level of alpha-synuclein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C220S | the mutant has in vitro ubiquitin hydrolase activity comparable to the wild-type protein | Homo sapiens |
S18Y | a naturally occuring polymorph, replacing the weak CKAA farnesylation motif with the optimal CVIM motif (S18Y/CVIM UCH-L1) leads to significantly increased accumulation of alpha-synuclein relative to S18Y | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | membrane associated form UCH-L1M, C-terminal farnesylation promotes the association of UCH-L1 with the membrane | Mus musculus | 5789 | - |
endoplasmic reticulum membrane | membrane associated form UCH-L1M, C-terminal farnesylation promotes the association of UCH-L1 with the membrane | Homo sapiens | 5789 | - |
membrane | membrane associated form UCH-L1M, C-terminal farnesylation promotes the association of UCH-L1 with cellular membranes, including the endoplasmic reticulum | Mus musculus | 16020 | - |
membrane | membrane associated form UCH-L1M, C-terminal farnesylation promotes the association of UCH-L1 with cellular membranes, including the endoplasmic reticulum | Homo sapiens | 16020 | - |
additional information | UCH-L1 exists in a membrane-associated form, UCH-L1M, in addition to the commonly studied soluble form | Mus musculus | - |
- |
additional information | UCH-L1 exists in a membrane-associated form, UCH-L1M, in addition to the commonly studied soluble form | Homo sapiens | - |
- |
plasma membrane | the membrane-associated UCH-L1M form is associated with the plasma membrane of oocytes, C-terminal farnesylation promotes the association of UCH-L1 with the membrane | Homo sapiens | 5886 | - |
plasma membrane | the membrane-associated UCH-L1M form, C-terminal farnesylation promotes the association of UCH-L1 with the membrane | Mus musculus | 5886 | - |
soluble | soluble UCH-L1 form, UCH-L1S | Mus musculus | - |
- |
soluble | soluble UCH-L1 form, UCH-L1S | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the proteasomal pathway is partially inhibited by UCH-L1M | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | C-terminal farnesylation promotes the association of UCH-L1 with membranes. Inhibition of UCH-L1 farnesylation by farnesyltransferase inhibitor FTI-277 | Mus musculus |
lipoprotein | C-terminal farnesylation promotes the association of UCH-L1 with membranes. Inhibition of UCH-L1 farnesylation by farnesyltransferase inhibitor FTI-277 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | cortex, UCH-L1M omprises about 30% of total UCH-L1 in diseased and normal human brain | Homo sapiens | - |
brain | in mouse brain regions, e.g. cortex, hippocampus, striatum, and midbrain, both UCH-L1M and UCH-L1S occur in varying relative amounts | Mus musculus | - |
brain cortex | - |
Mus musculus | - |
corpus striatum | - |
Mus musculus | - |
hippocampus | - |
Mus musculus | - |
midbrain | - |
Mus musculus | - |
neuron | primary dopaminergic neurons | Mus musculus | - |
oocyte | UCH-L1 is associated with the plasma membrane of oocytes | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the proteasomal pathway is partially inhibited by UCH-L1M | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase-L1 | - |
Mus musculus |
ubiquitin C-terminal hydrolase-L1 | - |
Homo sapiens |
UCH-L1 | - |
Mus musculus |
UCH-L1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the proteasomal pathway is partially inhibited by UCH-L1M | Homo sapiens |
additional information | membrane-associated farnesylated UCH-L1 promotes alpha-synuclein neurotoxicity, mechanism, overview | Mus musculus |
additional information | membrane-associated farnesylated UCH-L1 promotes alpha-synuclein neurotoxicity, mechanism, overview | Homo sapiens |