Cloned (Comment) | Organism |
---|---|
two plasmids with two types of YUH1 with the 6 histidine tag at the N-terminus (H6YUH1) or C-terminus (YUH1H6) expressed in Escherichia coli DH5alpha | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity chromatography, to more than 90% homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.61 | - |
YUH1H6 | Saccharomyces cerevisiae |
1.72 | - |
H6YUH1 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | H6YUH1 is unable to hydrolyze the linkage of ubiquitin with human growth hormone | Saccharomyces cerevisiae | ? | - |
? | |
Z-Arg-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase 1 | - |
Saccharomyces cerevisiae |
YUH1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
H6YUH1 shows 3.2 and 1.4 h of half lives at 4°C and 52°C, respectively. Immobilized YUH1 in 50% glycerol solution at -20°C results in 52% of decrease in specific activity for 7 days, corresponding to a 2.7fold increase compared with that of free YUH1 incubated in the same solution at 4°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Saccharomyces cerevisiae |