Application | Comment | Organism |
---|---|---|
medicine | Parkinson disease-associated UCH-L1 is regulated by reversible monoubiquitination involving auto-deubiquitination | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
COS-7 cells co-transfected with plasmids encoding His-tagged UCH-L1 and FLAG-tagged ubiquitin | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C90S | increases cellular monoubiquitin levels, 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated | Homo sapiens |
C90S/K157R | reduces monoubiquitination | Homo sapiens |
D30K | deficient in ubiquitin binding, is unable to increase cellular monoubiquitin levels | Homo sapiens |
H161D | 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated | Homo sapiens |
I93M | approximately doubles the monoubiquitination level of UCH-L1 | Homo sapiens |
additional information | fusion protein UbVV/L8A-UCH-L1 contains an L8A mutation in the ubiquitin moiety. Efficiently binds ubiquitin, increases cellular monoubiquitin levels | Homo sapiens |
S18Y | does not affect cellular levels of monoubiquitinated UCH-L1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | prevents deubiquitination | Homo sapiens | |
additional information | monoubiquitination inhibits ubiquitin binding in vitro and prevents UCH-L1 from regulating free ubiquitin levels in cells involving auto-deubiquitination. Intramolecular deubiquitination, which is dependent on the hydrolytic activity of UCH-L1, regulates the lifetime of monoubiquitination, thereby affecting the cellular level of monoubiquitinated UCH-L1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
additional information | perinucleus | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA resin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase-L1 | - |
Homo sapiens |
UCH-L1 | - |
Homo sapiens |