Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as GST-tagged enzyme | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D348A | site-directed mutagenesis, inactive mutant, leads to accumulation of ubiquitin on endosomes and the concomitant stabilization of an ubiquinated form of the signal transducing adeptor molecule, STAM | Homo sapiens |
additional information | enzyme downregulation in HeLa cells by siRNA, enzyme inhibition influences the degradation of the EGF receptor | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is inhibited by zinc-chelating agents | Homo sapiens | |
NEM | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | associated to | Homo sapiens | 5768 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | AMSH contains the JAMM motif, i.e. JAB1/MPN/Mov34 metalloenzyme motif, in the active site | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | AMSH is a deubiquinating enzyme with functions at the endosome, it opposes the ubiquitin-dependent sorting of receptors to lysosomes, the enzyme influences the degradation of the EGF receptor by association to endosomes, model of the enzyme's physiological function | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
K48-linked tetraubiquitin + H2O | - |
Homo sapiens | K48-linked diubiquitin + diubiquitin | - |
? | |
K63-linked polyubiquitin + H2O | - |
Homo sapiens | K63-linked polyubiquitin + ubiquitin | - |
? | |
additional information | AMSH is a deubiquinating enzyme with functions at the endosome, it opposes the ubiquitin-dependent sorting of receptors to lysosomes, the enzyme influences the degradation of the EGF receptor by association to endosomes, model of the enzyme's physiological function | Homo sapiens | ? | - |
? | |
additional information | AMSH interacts with the signal transducing adaptor molecule, STAM, via the SH3-domains | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AMSH contains the JAMM motif, i.e. JAB1/MPN/Mov34 metalloenzyme motif, in the active site | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AMSH | - |
Homo sapiens |
associated molecule with the SH3-domain of STAM | - |
Homo sapiens |
ubiquitin isopeptidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.3 | broad optimum | Homo sapiens |