Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | Ki: 0.035 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+ | Saccharomyces cerevisiae | |
Ag+ | complete inhibition at a concentration of 1 mM | Saccharomyces cerevisiae | |
diisopropylphosphorofluoridate | no inhibition | Saccharomyces cerevisiae | |
EDTA | Ki: 0.013 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+, Zn2+ and Co2+, at a concentration of 0.1 mM, are most effecient with 88% and 62% reactivation | Saccharomyces cerevisiae | |
iodoacetamide | 90% inhibition at a concentration of 5 mM | Saccharomyces cerevisiae | |
iodoacetate | 40% inhibition at a concentration of 5 mM, irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | can reverse the inhibition by chelating agents | Saccharomyces cerevisiae | |
Co2+ | can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 62%, inhibits the enzyme at higher concentrations, 10 mM | Saccharomyces cerevisiae | |
Mn2+ | can reverse the inhibition by chelating agents | Saccharomyces cerevisiae | |
Zn2+ | can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 88%, inhibits the enzyme at higher concentrations, 10 mM | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
400fold | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
230 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Gly-Gly + H2O | 7.2% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | ? | - |
? | |
benzoyl-Gly-Lys + H2O | 2% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | ? | - |
? | |
benzyloxycarbonyl-Ala-Arg(NO2) + H2O | 21% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Ala + nitroarginine | - |
? | |
Benzyloxycarbonyl-Ala-Phe + H2O | 10% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | Benzyloxycarbonyl-Ala + Phe | - |
? | |
Benzyloxycarbonyl-Glu-Tyr + H2O | 1.1% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
benzyloxycarbonyl-Gly-Glu + H2O | 31% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Glu | - |
? | |
benzyloxycarbonyl-Gly-Gly + H2O | 13% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Gly | - |
? | |
Benzyloxycarbonyl-Gly-Leu + H2O | best substrate | Saccharomyces cerevisiae | Benzyloxycarbonyl-Gly + Leu | - |
? | |
Benzyloxycarbonyl-Gly-Phe + H2O | 31% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | Benzyloxycarbonyl-Gly + Phe | - |
? | |
benzyloxycarbonyl-Phe-Leu + H2O | 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Leu | - |
? | |
benzyloxycarbonyl-Phe-Met + H2O | 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Met | - |
? | |
benzyloxycarbonyl-Phe-Phe + H2O | 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Phe | - |
? | |
dibenzyloxycarbonyl-Lys-Leu + H2O | 5.2% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | dibenzyloxycarbonyl-Lys + Leu | - |
? | |
Gly-Leu-Gly + H2O | 3% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | ? | - |
? | |
Gly-Leu-Tyr + H2O | 12% of the activity compared to benzyloxycarbonyl-Gly-Leu | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no hydrolysis of benzyloxycarbonyl-Leu-Leu, benzyloxycarbonyl-Leu-Phe, dibenzyloxycarbonyl-Lys-Phe, benzyloxycarbonyl-Phe-Ser, benzyloxycarbonyl-His-Leu, benzyloxycarbonyl-Pro-Try, benzyloxycarbonyl-Gly-Gly-Leu, benzyloxycarbonyl-Gly-Gly-Phe, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzyloxycarbonyl-Ala-Phe-NH2, benzyloxycarbonyl-Phe-Leu-NH2, benzyloxycarbonyl-Phe-Leu-NH2 and Gly-Leu | Saccharomyces cerevisiae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.2 | benzyloxycarbonyl-Gly-Leu as substrate, in presence of 0.5 M NaCl | Saccharomyces cerevisiae |