Application | Comment | Organism |
---|---|---|
drug development | potential target for drug development | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1 Blue cells as catalytically active poly-His-tagged recombinant enzyme. GST-fusion proteins expressed in Escherichia coli BL21 Codon Plus (DE3) cells | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 1 mM 92.2% activity relative to control | Trypanosoma cruzi | |
Co2+ | at 0.01 mM 59.7% activity relative to control | Trypanosoma cruzi | |
EDTA | 10 mM strongly inhibits | Trypanosoma cruzi | |
Mg2+ | at 1 mM 88% activity relative to control | Trypanosoma cruzi | |
Mn2+ | at 0.1 mM 76% activity relative to control | Trypanosoma cruzi | |
additional information | PMSF at 2 mM and E-64 at 0.001 mM have no effect on activity | Trypanosoma cruzi | |
Ni2+ | at 0.01 mM 44.6% activity relative to control | Trypanosoma cruzi | |
o-phenanthroline | 1 mM strongly inhibits | Trypanosoma cruzi | |
Zn2+ | at 0.01 mM 8.98% activity relative to control | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
0.166 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the absence of Co2+ | Trypanosoma cruzi | |
0.169 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the presence of Co2+ | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57700 | - |
x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
59000 | - |
x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
128000 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q6ZXC0 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and two ion-exchange chromatography steps (Ni- and Co-NTA resin), 20.4fold purification and 32% recovery | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
additional information | present in all life cycle stages | Trypanosoma cruzi | - |
trypomastigote | cell-derived and metacylic trypomastigotes | Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
cell-free extract | Trypanosoma cruzi |
85 | - |
20.4fold purified enzyme | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MCP-1 acts best on substrates having a basic amino acid at P1' position. Activity is very low with hydrophobic C-terminal residues | Trypanosoma cruzi | ? | - |
? | |
N-(3-[2-furyl]acryloyl)-Ala-Lys + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O | - |
Trypanosoma cruzi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
MCP-1 | - |
Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
residual activity after 5 min of incubation at 50°C is 21.4%, the enzyme is not thermostable | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
5.7 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the presence of Co2+ | Trypanosoma cruzi | |
20 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the absence of Co2+ | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.3 | - |
Trypanosoma cruzi |