BRENDA - Enzyme Database show
show all sequences of 3.4.17.15

Comparative study on activation mechanism of carboxypeptidase A1, A2 and B: first insights from steered molecular dynamics simulations

Jitonnom, J.; Sontag, C.; J. Mol. Graph. Model. 38, 298-303 (2012)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P48052
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
in silico conversion of zymogen into the primary cleavage state using available X-ray structures to investigate its spontaneous dissociation process of the prosegment from its associated enzyme domain using steered molecular dynamics simulation. The cleavage substantially destabilizes most of the hydrogen bonds at the prosegment-enzyme interface. During prosegment unbinding, the enzyme shows first rupture in the globular domain and then in the connecting segment
Homo sapiens
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
in silico conversion of zymogen into the primary cleavage state using available X-ray structures to investigate its spontaneous dissociation process of the prosegment from its associated enzyme domain using steered molecular dynamics simulation. The cleavage substantially destabilizes most of the hydrogen bonds at the prosegment-enzyme interface. During prosegment unbinding, the enzyme shows first rupture in the globular domain and then in the connecting segment
Homo sapiens
Other publictions for EC 3.4.17.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732350
Jitonnom
Comparative study on activatio ...
Homo sapiens
J. Mol. Graph. Model.
38
298-303
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732609
Pereira
Carboxypeptidases A1 and A2 fr ...
Rattus norvegicus
Peptides
33
67-76
2012
-
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668948
Liu
Cloning and characterization o ...
Mayetiola destructor
Insect Biochem. Mol. Biol.
36
665-673
2006
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668310
Srivastava
Molecular cloning and cDNA seq ...
Paralichthys olivaceus
Comp. Biochem. Physiol. B
135
593-599
2003
1
-
1
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
669813
Bentley
The imprinted region on human ...
Homo sapiens
J. Med. Genet.
40
249-256
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670816
Jimenez
NMR solution structure of the ...
Homo sapiens
Protein Sci.
12
296-305
2003
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667459
Uratani
Latexin, a carboxypeptidase A ...
Rattus norvegicus
Biochem. J.
346
817-826
2000
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668583
Villanueva
Hydrogen exchange monitored by ...
Homo sapiens
FEBS Lett.
472
27-33
2000
-
-
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
669188
Reverter
Overexpression of human procar ...
Homo sapiens
J. Biol. Chem.
273
3535-3541
1998
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
2
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
647257
Reverter
Characterization and prelimina ...
Homo sapiens
FEBS Lett.
420
7-10
1997
-
-
1
1
-
-
-
8
-
-
-
-
-
4
-
1
1
-
-
1
-
-
8
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
8
-
-
-
-
-
-
1
1
-
1
-
-
8
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
667298
Laethem
Expression and characterizatio ...
Homo sapiens
Arch. Biochem. Biophys.
332
8-18
1996
-
-
1
-
-
-
-
3
-
-
1
-
-
5
-
1
1
-
-
1
3
-
4
1
1
-
1
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
1
-
1
3
-
4
1
1
-
1
3
1
-
-
-
-
-
-
-
-
-
586913
Gardell
A novel rat carboxypeptidase, ...
Rattus norvegicus
J. Biol. Chem.
263
17828-17836
1988
-
-
1
-
-
-
-
4
-
-
-
-
-
3
-
-
1
-
-
2
-
-
7
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
2
-
-
7
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-