Crystallization (Comment) | Organism |
---|---|
enzyme in complex with leech carboxypeptidase inhibitor, complexing in 50 mM Tris-HCl, pH 7.5, and 100 mM NaCl, at 20°C, complex purification by gel filtration, crystallization by mixing of equal volumes of protein, containing 10-12 mg/ml protein, and reservoir solutions, the latter containing 1.5 M lithium sulfate monohydrate and 100 mM Tris, pH 8.5, sitting drop vapour diffusion method, X-ray diffraction structure determination and analysis at 2.2 A resolution | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leech carboxypeptidase inhibitor | LCI, tight-binding, competitive inhibition, the inhibitor contains four disulfide bonds, biding structure with CPA, oxidative folding pathway and intermediate of wild-type and mutant C19A/C43A mutant, determination of thermodynamics and conformational stability of wild-type and mutant enzymes at pH 8.4/high concentration of DTT, overview | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis of enzyme in complex with leech carboxypeptidase inhibitor | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
CPA | - |
Bos taurus |