Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
Y248A | the ratio of turnover number to Km-value with hippuryl-L-Phe as substrate is 113fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with N-[3-(2-furyl)acryloyl]-Phe-Phe as substrate is 262fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate as substrate is 85fold lower than that of the wild-type enzyme. The Ki-value for (2R,3S)-2-benzyl-3,4-epoxybutanoic acid is 3.1fold higher than the wild-type value | Bos taurus |
Y248F | the ratio of turnover number to Km-value with hippuryl-L-Phe as substrate is 49fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with N-[3-(2-furyl)acryloyl]-Phe-Phe as substrate is 49fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate as substrate is 52.4fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with hippuryl-DL-beta-phenyllactate as substrate is 6.5fold lower than that of the wild-type enzyme.The Ki-value for (2R,3S)-2-benzyl-3,4-epoxybutanoic acid is 86% of the wild-type value | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
3.3 | - |
hippuryl-L-Phe | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
5.6 | - |
hippuryl-L-Phe | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
5.8 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
27 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
60 | - |
hippuryl-L-Phe | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
76 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
120 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
340 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
780 | - |
hippuryl-DL-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
1300 | - |
hippuryl-DL-beta-phenyllactate | pH 7.5, 25°C, wild-type enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-DL-beta-phenyllactate + H2O | - |
Bos taurus | ? | - |
? | |
hippuryl-L-Phe + H2O | - |
Bos taurus | hippuric acid + L-Phe | - |
? | |
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O | - |
Bos taurus | N-[3-(2-furyl)acryloyl]-Phe + Phe | - |
? | |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate + H2O | - |
Bos taurus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
0.1 | - |
hippuryl-DL-beta-phenyllactate | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
0.11 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
0.27 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
0.39 | - |
hippuryl-DL-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
0.55 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
0.56 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
0.88 | - |
hippuryl-L-Phe | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
1 | - |
N-[3-(2-furyl)acryloyl]-Phe-Phe | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
4 | - |
hippuryl-L-Phe | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
5.1 | - |
hippuryl-L-Phe | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
6.08 | - |
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus | |
6.08 | - |
hippuryl-L-Phe | pH 7.5, 25°C, wild-type enzyme | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid | pH 7.5, 25°C, mutant enzyme Y248F | Bos taurus | |
0.35 | - |
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid | pH 7.5, 25°C, wild-type enzyme | Bos taurus | |
1.1 | - |
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid | pH 7.5, 25°C, mutant enzyme Y248A | Bos taurus |