Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
General Stability | Organism |
---|---|
resistant against freezing and thawing | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Ala-Phe-CH2Cl | IC50: 0.06 mM | Rattus norvegicus | |
diisopropylfluorophosphate | IC50: 1.15 mM | Rattus norvegicus | |
Diprotin A | 0.1 mM, 50% inhibition | Rattus norvegicus | |
Hg2+ | 1 mM, complete inhibition, IC50: 0.67 mM | Rattus norvegicus | |
p-chloromercuribenzene sulfonic acid | IC50: 0.27 mM | Rattus norvegicus | |
pepstatin | 0.1 mM, 15% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 4.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus norvegicus | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
280000 | - |
non-denaturing PAGE | Rattus norvegicus |
290000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
bronchus | - |
Rattus norvegicus | - |
cerebellum | activity in neuroglia cells, no activity in small granule cells, Purkinje cells, remified dendrites and myelinated fibers | Rattus norvegicus | - |
cerebrum | - |
Rattus norvegicus | - |
epididymis | - |
Rattus norvegicus | - |
esophagus | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | activity in proximal tubules, distal tubules, collecting tubules and transitional cells, no activity in glomerulus and Bowmans capsule | Rattus norvegicus | - |
large intestine | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
ovary | activity in corpus luteum and corpus albicus, no activity in follicles | Rattus norvegicus | - |
pancreas | activity in ducts, no activity in acinar cells and islets of Langerhans | Rattus norvegicus | - |
prostate | - |
Rattus norvegicus | - |
salivary gland | activity in excretory duct and stereocilia, no activity in glandular acinus and intercalated portion | Rattus norvegicus | - |
seminal vesicle | - |
Rattus norvegicus | - |
small intestine | - |
Rattus norvegicus | - |
spleen | activity in red pulp, no activity in white pulp | Rattus norvegicus | - |
stomach | - |
Rattus norvegicus | - |
testis | activity in spermatogonia, spermatozoa, Sertoli cells and Leygig cells, no activity in basement cells | Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
trachea | - |
Rattus norvegicus | - |
uterus | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.583 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, pH 4.0, 50 mM sodium acetate buffer, stable for 10-14 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(pGlu)LYENKPRRRPYIL + H2O | i.e. neurotensin, 15.0% degradation after 16 h | Rattus norvegicus | (pGlu)L + YENKPRRRPYIL | - |
? | |
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | 33.8% degradation within 60 min | Rattus norvegicus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
DRVYIHPF + H2O | i.e. angiotensin II, 18.2% degradation after 16 h | Rattus norvegicus | DRV + YIHPF | - |
? | |
DRVYIHPFHL + H2O | i.e. angiotensin I, 11.3% degradation after 16 h | Rattus norvegicus | DRV + YIHPFHL | - |
? | |
GKPIPFFRLK + H2O | endo-type substrate, 24.5% degradation after 16 h | Rattus norvegicus | GKPIP + FFRLK | - |
? | |
GNLWATGHFM-NH2 + H2O | i.e. neuromedin B, complete degradation after 16 h | Rattus norvegicus | GNL + WATGHFM-NH2 | - |
? | |
additional information | substrates not cleaved after 24 h of digestion: dynorphin, beta-casomorphin-7, Leu-enkephalin, Met-enkephalin, neurokinin-A, LVV-hemorphin, bradykinin | Rattus norvegicus | ? | - |
? | |
additional information | an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 | Rattus norvegicus | ? | - |
? | |
RVYIHPF + H2O | i.e. angiotensin III, 95.0% degradation after 15 min | Rattus norvegicus | RVY + IHPF | - |
? | |
YGGFLRKYP + H2O | i.e. beta-neo-endorphin, 28% degradation after 16 h | Rattus norvegicus | YGG + FLRKYP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.1 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 4.0 | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | - |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | stable | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.06 | - |
IC50: 0.06 mM | Rattus norvegicus | Ala-Ala-Phe-CH2Cl | |
0.27 | - |
IC50: 0.27 mM | Rattus norvegicus | p-chloromercuribenzene sulfonic acid | |
0.67 | - |
1 mM, complete inhibition, IC50: 0.67 mM | Rattus norvegicus | Hg2+ | |
1.15 | - |
IC50: 1.15 mM | Rattus norvegicus | diisopropylfluorophosphate |