Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Streptococcus thermophilus | |
iodoacetate | - |
Streptococcus thermophilus | |
phenylmethylsulfonyl fluoride | - |
Streptococcus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
Gly-Pro-p-nitroanilide | - |
Streptococcus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Streptococcus thermophilus |
160000 | - |
gel filtration | Streptococcus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus thermophilus | - |
ACA-DC 4 | - |
Streptococcus thermophilus ACA-DC 4 | - |
ACA-DC 4 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
285 | - |
- |
Streptococcus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-4-nitroanilide + H2O | 1% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala + 4-nitroaniline | - |
? | |
Ala-Pro-Gly + H2O | 72% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala-Pro + Gly | - |
? | |
Arg-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg + 4-nitroaniline | - |
? | |
Arg-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Arg + 4-nitroaniline | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg-Pro + 4-nitroaniline | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Phe-4-nitroanilide + H2O | 1% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Gly-Phe + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus thermophilus | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus thermophilus ACA-DC 4 | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-Arg-Pro + H2O | as active as with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? | |
Gly-Pro-Arg-Pro + H2O | as active as with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | ? | - |
? | |
Ile-Pro-Ile + H2O | 34% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? | |
Phe-4-nitroanilide + H2O | 7% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Phe + 4-nitroaniline | - |
? | |
Phe-4-nitroanilide + H2O | 7% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus ACA-DC 4 | Phe + 4-nitroaniline | - |
? | |
Pro-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Pro + 4-nitroaniline | - |
? | |
Val-Pro-Leu + H2O | 83% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Streptococcus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptococcus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | 10°C: 17% of maximal activity, about about 40% of maximal activity at 25°C and at 60°C | Streptococcus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, stable | Streptococcus thermophilus |
60 | - |
1 min, in absence of substrate, 96% loss of activity | Streptococcus thermophilus |
70 | - |
1 min, complete loss of activity | Streptococcus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 15% of maximal activity, pH 9.0: about 85% of maximal activity | Streptococcus thermophilus |