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Literature summary for 3.4.14.4 extracted from

  • Salopek-Sondi, B.; Vukelic, B.; Spoljaric, J.; Simaga, S.; Vujaklija, D.; Makarevic, J.; Jajcanin, N.; Abramic, M.
    Functional tyrosine residue in the active center of human dipeptidyl peptidase III (2008), Biol. Chem., 389, 163-167.
    View publication on PubMed
Search for more literature for 3.4.14.4

Cloned(Commentary)

Cloned (Comment) Organism
into the pET21b vector for expression in Escherichia coli BL21DE3RIL+ cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y318F the potential functional role of the well-conserved tyrosine 318 residue in the active center of human DPP III is investigated Homo sapiens
Y395F the potential functional role of the well-conserved tyrosine 395 residue in the active center of human DPP III is investigated Homo sapiens
Y644F the potential functional role of the well-conserved tyrosine 644 residue in the active center of human DPP III is investigated Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Tyr-Phe-NHOH
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 Arg-Arg-2-naphthylamide mutant Y644F Homo sapiens
0.0084
-
 Arg-Arg-2-naphthylamide wild-type enzyme Homo sapiens
0.0104
-
 Arg-Arg-2-naphthylamide mutant Y318F Homo sapiens
0.0114
-
 Arg-Arg-2-naphthylamide mutant Y395F Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 predicted, and verified by SDS-PAGE and Western Blot analysis, purified human erythtocyte DPP III and recombinant DPP III Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NY33
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration on a Sephacryl S-200 column, followed by FPLC on a Mono Q column, recombinant and human erythtocyte DPP III is purified Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Arg-2-naphthylamide + H2O substrate used in the activity assay Homo sapiens Arg-Arg + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase III
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
 Arg-Arg-2-naphthylamide mutant Y318F Homo sapiens
8
-
 Arg-Arg-2-naphthylamide mutant Y395F Homo sapiens
27.3
-
 Arg-Arg-2-naphthylamide mutant Y644F Homo sapiens
31.5
-
 Arg-Arg-2-naphthylamide wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 activity assay Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000146
-
 Tyr-Phe-NHOH wild-type DPP III at pH 8.0 Homo sapiens
0.000234
-
 Tyr-Phe-NHOH mutant Y318F at pH 8.0 Homo sapiens