Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.14.12 extracted from

  • Xu, Y.; Nakajima, Y.; Ito, K.; Zheng, H.; Oyama, H.; Heiser, U.; Hoffmann, T.; Gaertner, U.T.; Demuth, H.U.; Yoshimoto, T.
    Novel inhibitor for prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis and details of substrate-recognition mechanism (2008), J. Mol. Biol., 375, 708-719.
    View publication on PubMed
Search for more literature for 3.4.14.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Porphyromonas gingivalis
into the pQE30 vector for transformation of Escherichia coli M15 cells Porphyromonas gingivalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, with 100 mM Ches (pH 9.0), 1.1 M potassium/sodium tartrate, 200 mM lithium sulfate Porphyromonas gingivalis
the structure of the prolyl tripeptidyl aminopeptidase complexed with the inhibitor is determined at 2.2 A resolution, the structure of the mutant E636A at 2.0 A, and in complex with the inhibitor at 1.95 A resolution Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
E205A mutant for examination of the function of the glutamate residue in substrate recognition, mutant expressed in inclusion bodies Porphyromonas gingivalis
E205Q mutant for examination of the function of the glutamate residue in substrate recognition, mutant expressed in soluble form, no activity detected Porphyromonas gingivalis
E636A reduced activity Porphyromonas gingivalis
E636A mutant for examination of the function of the glutamate residue in substrate recognition Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ile-pyrrolidin-2-yl boronic acid
-
 Porphyromonas gingivalis
Ala-Ile-pyrrolidine-2-yl boronic acid
-
 Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 Gly-Ala-Pro-2-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.17
-
 Ala-Phe-Pro-beta-naphthylamide E636A mutant Porphyromonas gingivalis
0.38
-
 Gly-Ala-Pro-2-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.38
-
 Gly-Ala-Pro-beta-naphthylamide prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues Porphyromonas gingivalis
0.42
-
 Gly-Ala-Pro-2-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.42
-
 Gly-Ala-Pro-beta-naphthylamide prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues Porphyromonas gingivalis
0.85
-
 Gly-Ala-Pro-2-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.85
-
 Ala-Phe-Pro-beta-naphthylamide E636A mutant Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79390
-
-
 Porphyromonas gingivalis
79390
-
 calculated from amino acid sequence Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis Q7MUW6
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyoperal HW650C chromatography and DEAE-Toyopearl column chromatography Porphyromonas gingivalis
using a Toyopearl HW650C and a DEAE-Toyopearl column Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Phe-Pro-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Ala-Phe-Pro + 2-naphthylamine
-
 ?
Ala-Phe-Pro-beta-naphthylamide + H2O
-
 Porphyromonas gingivalis Ala-Phe-Pro + beta-naphthylamine
-
 ?
Gly-Ala-Pro-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Gly-Ala-Pro + 2-naphthylamine
-
 ?
Gly-Ala-Pro-beta-naphthylamide + H2O
-
 Porphyromonas gingivalis Gly-Ala-Pro + beta-naphthylamine
-
 ?
Z-Gly-Ala-Pro-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Z-Gly-Ala-Pro + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
prolyl tripeptidyl aminopeptidase
-
 Porphyromonas gingivalis
PTP
-
 Porphyromonas gingivalis
PTP39
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.65
-
 Gly-Ala-Pro-2-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
2.65
-
 Gly-Ala-Pro-beta-naphthylamide prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues Porphyromonas gingivalis
6.8
-
 Gly-Ala-Pro-2-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
6.8
-
 Ala-Phe-Pro-beta-naphthylamide E636A mutant Porphyromonas gingivalis
354
-
 Gly-Ala-Pro-2-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
354
-
 Gly-Ala-Pro-beta-naphthylamide prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues Porphyromonas gingivalis
511
-
 Gly-Ala-Pro-2-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
511
-
 Ala-Phe-Pro-beta-naphthylamide E636A mutant Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity assay Porphyromonas gingivalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000881
-
 Ala-Ile-pyrrolidin-2-yl boronic acid wild type enzyme, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.0000881
-
 Ala-Ile-pyrrolidin-2-yl boronic acid prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues Porphyromonas gingivalis
0.0488
-
 Ala-Ile-pyrrolidin-2-yl boronic acid mutant enzyme E636A, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C Porphyromonas gingivalis
0.0488
-
 Ala-Ile-pyrrolidin-2-yl boronic acid mutant E636A Porphyromonas gingivalis