Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | 5.0 mM, 36% inhibition | Prevotella nigrescens | |
diisopropyl fluorophosphate | 5.0 mM, 69% inhibition | Prevotella nigrescens | |
additional information | 1 mM NEM, 5 mM 1,10-phenanthroline, 1 mM EDTA, 0.2 mM E64, 0.4 mM antipain, 0.2 mM leupeptin, 1 mg/ml aprotinin, 0.2 mM bestatin or 1 mM mercaptoethanol; no inhibition by | Prevotella nigrescens | |
Pefabloc SC | 1.0 mM, 33% inhibition | Prevotella nigrescens | |
tosyl-L-lysine chloromethyl ketone | 1.0 mM, 47% inhibition | Prevotella nigrescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
Ala-Ala-Pro-p-nitroanilide | pH 7.5, 37°C | Prevotella nigrescens | |
0.084 | - |
Lys-Pro-p-nitroanilide | pH 7.5, 37°C | Prevotella nigrescens | |
0.114 | - |
Arg-Pro-p-nitroanilide | pH 7.5, 37°C | Prevotella nigrescens | |
0.129 | - |
Ala-Phe-Pro-p-nitroanilide | pH 7.5, 37°C | Prevotella nigrescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Prevotella nigrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prevotella nigrescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Prevotella nigrescens |
Storage Stability | Organism |
---|---|
-40°C, stable for several weeks | Prevotella nigrescens |
4°C, stable for 24 h | Prevotella nigrescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Pro-p-nitroanilide + H2O | - |
Prevotella nigrescens | Ala-Ala-Pro + p-nitroaniline | - |
? | |
Ala-Phe-Pro-p-nitroanilide + H2O | 66% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Ala-Phe-Pro + p-nitroaniline | - |
? | |
Ala-Pro-p-nitroanilide + H2O | 13% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Ala-Pro + p-nitroaniline | - |
? | |
Arg-Pro-p-nitroanilide + H2O | 22% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Arg-Pro + p-nitroaniline | - |
? | |
Gly-Pro-p-nitroanilide + H2O | 3% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Gly-Pro + p-nitroaniline | - |
? | |
Lys-Pro-p-nitroanilide + H2O | 24% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Lys-Pro + p-nitroaniline | - |
? | |
additional information | no activity with Ala-Phe-p-nitroanilide, Ala-Ala-p-nitroanilide, Ala-p-nitroanilide, Pro-p-nitroanilide, benzoyl-Arg-p-nitroanilide, succinyl-Ala-Ala-Ala-p-nitroanilide, succinyl-Ala-Ala-Pro-p-nitroanilide, tosyl-Gly-Pro-Lys-4-nitroanilide | Prevotella nigrescens | ? | - |
? | |
Phe-Pro-p-nitroanilide + H2O | 13% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Phe-Pro + p-nitroaniline | - |
? | |
Ser-Pro-p-nitroanilide + H2O | 13% of the activity with Ala-Ala-Pro-p-nitroanilide | Prevotella nigrescens | Ser-Pro + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Prevotella nigrescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, complete loss of activity | Prevotella nigrescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Prevotella nigrescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | no significant activity is observed below pH 6.0 and above pH 8.5 | Prevotella nigrescens |