Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.21 extracted from

  • Kong, X.; Liu, Y.; Gou, X.; Zhu, S.; Zhang, H.; Wang, X.; Zhang, J.
    Directed evolution of alpha-aspartyl dipeptidase from Salmonella typhimurium (2001), Biochem. Biophys. Res. Commun., 289, 137-142.
    View publication on PubMed
Search for more literature for 3.4.13.21

Protein Variants

Protein Variants Comment Organism
N89H/Q153E/L205R 47fold increase in enzyme activity, increased stability Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.26
-
 L-Asp-4-nitroanilide mutant N89H/Q153E/L205R Salmonella enterica subsp. enterica serovar Typhimurium
3.98
-
 L-Asp-4-nitroanilide wild-type Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp-4-nitroanilide + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-Asp + 4-nitroaniline
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, 25% residual activity for wild-type, 80% residual activity for mutant N89H/Q153E/L205R Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 L-Asp-4-nitroanilide wild-type Salmonella enterica subsp. enterica serovar Typhimurium
5.83
-
 L-Asp-4-nitroanilide mutant N89H/Q153E/L205R Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 mutant N89H/Q153E/L205R Salmonella enterica subsp. enterica serovar Typhimurium
7.2
-
 wild-type Salmonella enterica subsp. enterica serovar Typhimurium