Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus onnurineus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.5 mM | Thermococcus onnurineus | |
EDTA | 0.5 mM, complete inhibition | Thermococcus onnurineus | |
Mn2+ | 0.5 mM | Thermococcus onnurineus | |
Ni2+ | 0.5 mM | Thermococcus onnurineus | |
Zn2+ | 0.5 mM, complete inhibition | Thermococcus onnurineus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
0.8 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
1.1 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.5 mM Co2+ stimulates activity 4.8fold, the enzyme contains 0.09 mol of Co2+ per mol of monomer | Thermococcus onnurineus | |
NaCl | stimulates, optimal cobcentration | Thermococcus onnurineus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37900 | - |
x * 37900, His-tagged enzyme, SDS-PAGE | Thermococcus onnurineus |
71000 | - |
dimeric form prevalent in presence of 0.1 mM Co2+, gel filtration | Thermococcus onnurineus |
600000 | - |
oligomeric form prevalent in absence of Co2+, gel filtration | Thermococcus onnurineus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus onnurineus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucyl 4-nitroanilide + H2O | catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide | Thermococcus onnurineus | L-leucine + 4-nitroaniline | - |
? | |
L-methionyl 4-nitroanilide + H2O | - |
Thermococcus onnurineus | L-methionine + 4-nitroaniline | - |
? | |
N-acetyl-L-leucyl 4-nitroanilide + H2O | catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide | Thermococcus onnurineus | N-acetyl-L-leucine + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37900, His-tagged enzyme, SDS-PAGE | Thermococcus onnurineus |
Synonyms | Comment | Organism |
---|---|---|
deblocking aminopeptidase | ambiguous | Thermococcus onnurineus |
TNA1-DAP | - |
Thermococcus onnurineus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 100 | - |
Thermococcus onnurineus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | 70°C: about 35% of maximal activity, 90-100°C: optimum | Thermococcus onnurineus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
96 | - |
1 h, t1/2 is less than 10 min in 50 mM HEPES buffer (pH 7.5) in absence of CoCl2, stable for at least 1 h in presence of 0.1 mM CoCl2 | Thermococcus onnurineus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
10.9 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
37.5 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermococcus onnurineus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.5: about 70% of maximal activity (HEPES buffer), about 40% of maximal activity (Tris-HCl buffer) | Thermococcus onnurineus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
47 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
55 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus |