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Literature summary for 3.4.11.6 extracted from

  • Fukasawa, K.M.; Hirose, J.; Hata, T.; Ono, Y.
    Aspartic acid 405 contributes to the substrate specificity of aminopeptidase B (2006), Biochemistry, 45, 11425-11431.
    View publication on PubMed
Search for more literature for 3.4.11.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D315N decreased activity compared to the wild type enzyme Rattus norvegicus
D405N decreased activity compared to the wild type enzyme Rattus norvegicus
D406N decreased activity compared to the wild type enzyme Rattus norvegicus
E256Q decreased activity compared to the wild type enzyme Rattus norvegicus
E260Q decreased activity compared to the wild type enzyme Rattus norvegicus
E267Q decreased activity compared to the wild type enzyme Rattus norvegicus
E268Q decreased activity compared to the wild type enzyme Rattus norvegicus
E300Q no activity Rattus norvegicus
E387Q increased activity compared to the wild type enzyme Rattus norvegicus
E388Q decreased activity compared to the wild type enzyme Rattus norvegicus
E410Q decreased activity compared to the wild type enzyme Rattus norvegicus
E414Q decreased activity compared to the wild type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00003
-
 L-Arg-2-naphthylamide mutant enzyme D406N Rattus norvegicus
0.000062
-
 L-Arg-2-naphthylamide mutant enzyme E26oQ Rattus norvegicus
0.000064
-
 L-Arg-2-naphthylamide mutant enzyme E268Q Rattus norvegicus
0.000088
-
 L-Arg-2-naphthylamide wild type enzyme Rattus norvegicus
0.000099
-
 L-Arg-2-naphthylamide mutant enzyme E256Q Rattus norvegicus
0.000105
-
 L-Arg-2-naphthylamide mutant enzyme D405E Rattus norvegicus
0.000109
-
 L-Arg-2-naphthylamide mutant enzyme E387Q Rattus norvegicus
0.000111
-
 L-Arg-2-naphthylamide mutant enzyme E388Q Rattus norvegicus
0.000119
-
 L-Arg-2-naphthylamide mutant enzyme E267Q Rattus norvegicus
0.000123
-
 L-Arg-2-naphthylamide mutant enzyme D405N Rattus norvegicus
0.000124
-
 L-Tyr-2-naphthylamide mutant enzyme D405A Rattus norvegicus
0.000125
-
 L-Arg-2-naphthylamide mutant enzyme D315N Rattus norvegicus
0.000128
-
 L-Arg-2-naphthylamide mutant enzyme E410Q Rattus norvegicus
0.00014
-
 L-Lys-2-naphthylamide wild type enzyme Rattus norvegicus
0.000197
-
 L-Tyr-2-naphthylamide wild type enzyme Rattus norvegicus
0.000213
-
 L-Lys-2-naphthylamide mutant enzyme D405E Rattus norvegicus
0.000384
-
 L-Arg-2-naphthylamide mutant enzyme E414Q Rattus norvegicus
0.000578
-
 L-Arg-2-naphthylamide mutant enzyme D405A Rattus norvegicus
0.0169
-
 L-Lys-2-naphthylamide mutant enzyme D405A Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72550
-
 deduced from amino acid sequence Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B column chromatography and Sephadex G-150 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-2-naphthylamide + H2O less than 0.02% activity compared to L-Arg-2-naphthylamide Rattus norvegicus Gly + 2-naphthylamine
-
 ?
L-Ala-2-naphthylamide + H2O 0.1% activity compared to L-Arg-2-naphthylamide Rattus norvegicus L-Ala + 2-naphthylamine
-
 ?
L-Arg-2-naphthylamide + H2O 100% activity Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?
L-Lys-2-naphthylamide + H2O 41% activity compared to L-Arg-2-naphthylamide Rattus norvegicus L-Lys + 2-naphthylamine
-
 ?
L-Phe-2-naphthylamide + H2O 0.171% activity compared to L-Arg-2-naphthylamide Rattus norvegicus L-Phe + 2-naphthylamine
-
 ?
L-Tyr-2-naphthylamide + H2O 0.171% activity compared to L-Arg-2-naphthylamide Rattus norvegicus L-Tyr + 2-naphthylamine
-
 ?
L-Val-2-naphthylamide + H2O 0.034% activity compared to L-Arg-2-naphthylamide Rattus norvegicus L-Val + 2-naphthylamine
-
 ?
additional information no detectable activity with L-Asp-2-naphthylamide Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
APB
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037 0.23 L-Lys-2-naphthylamide mutant enzyme D405E Rattus norvegicus
0.49
-
 L-Arg-2-naphthylamide mutant enzyme D405A Rattus norvegicus
0.83
-
 L-Lys-2-naphthylamide mutant enzyme D405A Rattus norvegicus
1
-
 L-Tyr-2-naphthylamide mutant enzyme D405A Rattus norvegicus
2.1
-
 L-Tyr-2-naphthylamide wild type enzyme Rattus norvegicus
2.1
-
 L-Arg-2-naphthylamide mutant enzyme D405N Rattus norvegicus
6.3
-
 L-Arg-2-naphthylamide mutant enzyme E410Q Rattus norvegicus
11.5
-
 L-Arg-2-naphthylamide mutant enzyme D405E Rattus norvegicus
11.7
-
 L-Arg-2-naphthylamide mutant enzyme E268Q Rattus norvegicus
12
-
 L-Arg-2-naphthylamide mutant enzyme E26oQ Rattus norvegicus
13.5
-
 L-Arg-2-naphthylamide mutant enzyme E414Q Rattus norvegicus
19.6
-
 L-Arg-2-naphthylamide mutant enzyme D406N Rattus norvegicus
23.6
-
 L-Lys-2-naphthylamide wild type enzyme Rattus norvegicus
24.1
-
 L-Arg-2-naphthylamide mutant enzyme E267Q Rattus norvegicus
38
-
 L-Arg-2-naphthylamide mutant enzyme D315N Rattus norvegicus
51.8
-
 L-Arg-2-naphthylamide mutant enzyme E256Q Rattus norvegicus
53.3
-
 L-Arg-2-naphthylamide mutant enzyme E388Q Rattus norvegicus
54.1
-
 L-Arg-2-naphthylamide wild type enzyme Rattus norvegicus
60
-
 L-Arg-2-naphthylamide mutant enzyme E387Q Rattus norvegicus
79.3
-
 L-Lys-2-naphthylamide mutant enzyme D405E Rattus norvegicus