Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, with His-tag | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.005 mM, 5% residual activity | Streptomyces sp. | |
antipain | 0.074 mM, 46% residual activity | Streptomyces sp. | |
bestatin | 0.13 mM, 30% residual activity | Streptomyces sp. | |
Cd2+ | 1 mM, 9.2% residual activity | Streptomyces sp. | |
chymostatin | 0.1 mM, 35% residual activity | Streptomyces sp. | |
EDTA | 1 mM, 6% residual activity | Streptomyces sp. | |
pefabloc | 1 mM, 45% residual activity | Streptomyces sp. | |
Zn2+ | 1 mM, 36% residual activity | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-Lys-4-nitroanilide | native enzyme, pH 7.0, 37°C | Streptomyces sp. | |
0.11 | - |
L-Lys-4-nitroanilide | recombinant enzyme, pH 7.0, 37°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 149% of initial activity | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94986 | - |
1 * 95000, SDS-PAGE, 1 * 94986, calculated | Streptomyces sp. |
95000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. TH-4 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.3 | - |
pH 7.0, 37°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-L-Lys-4-nitroanilide + H2O | 21% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. | Boc-L-Lys + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | 40% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. | L-Arg + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | 40% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. TH-4 | L-Arg + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | 9.5% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | 9.5% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. TH-4 | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-L-Arg + H2O | 50fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Leu + L-Arg | - |
? | |
L-Leu-L-Arg + H2O | 50fold as active as L-Lys-L-Lys | Streptomyces sp. TH-4 | L-Leu + L-Arg | - |
? | |
L-Leu-L-Met + H2O | 49.4fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Leu + L-Met | - |
? | |
L-Leu-L-Met + H2O | 49.4fold as active as L-Lys-L-Lys | Streptomyces sp. TH-4 | L-Leu + L-Met | - |
? | |
L-Lys-4-nitroanilide + H2O | - |
Streptomyces sp. | L-Lys + 4-nitroaniline | - |
? | |
L-Lys-L-Arg + H2O | 25.5fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Lys + L-Arg | - |
? | |
L-Lys-L-Lys + H2O | - |
Streptomyces sp. | L-Lys | - |
? | |
L-Met-4-nitroanilide + H2O | 5.7% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. | L-Met + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | 6.8% of the activity with L-Lys-4-nitroanilide | Streptomyces sp. | L-Phe + 4-nitroaniline | - |
? | |
L-Phe-L-Arg + H2O | 51.8fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Phe + L-Arg | - |
? | |
L-Phe-L-Phe + H2O | 39.2fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Phe | - |
? | |
L-Tyr-L-Arg + H2O | best substrate among dipeptides, 57.9fold as active as L-Lys-L-Lys | Streptomyces sp. | L-Tyr + L-Arg | - |
? | |
additional information | no hydrolysis of Lys-Pro and Pro-Phe bond | Streptomyces sp. | ? | - |
? | |
additional information | no hydrolysis of Lys-Pro and Pro-Phe bond | Streptomyces sp. TH-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 95000, SDS-PAGE, 1 * 94986, calculated | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, 50% residual activity | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.7 | - |
L-Lys-4-nitroanilide | native enzyme, pH 7.0, 37°C | Streptomyces sp. | |
21.3 | - |
L-Lys-4-nitroanilide | recombinant enzyme, pH 7.0, 37°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces sp. |