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Literature summary for 3.4.11.1 extracted from

  • Pena-Diaz, P.; Vancova, M.; Resl, C.; Field, M.C.; Lukes, J.
    A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei (2017), PLoS Pathog., 13, e1006310 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
gene LAP, quantitative real-time PCR expression analysis, recombinant expression of V5-tagged TbLAP1 in Trypanosoma brucei, recombinant ectopic expression of HA-tagged TbLAP1 in procyclic Trypanosoma brucei Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information knockdown of TbLAP1 by RNAi resulting in a growth defect Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast the enzyme localizes to kinetoplast DNA and the nabelschnur, the proteinaceous link connecting progeny kDNAs at late stages of segregation Trypanosoma brucei brucei 20023
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additional information immunolocalization of in situ of V5-tagged TbLAP1 protein Trypanosoma brucei brucei
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Metals/Ions

Metals/Ions Comment Organism Structure
additional information LAP1 is a metallopeptidase Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q57W67
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Trypanosoma brucei brucei 927/4 GU Tat10.1 Q57W67
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Subunits

Subunits Comment Organism
homohexamer 6 * 70000, SDS-PAGE Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
LAP1
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 Trypanosoma brucei brucei
leucine aminopeptidase
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 Trypanosoma brucei brucei
leucine aminopeptidase 1
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 Trypanosoma brucei brucei
M17 family leucyl aminopeptidase metalloprotease
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 Trypanosoma brucei brucei
TbLAP1
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 Trypanosoma brucei brucei

General Information

General Information Comment Organism
evolution the enzyme belongs to the M17 family of proteases Trypanosoma brucei brucei
malfunction TbLAP1 knockdown results in delayed cytokinesis and ectopic expression leading to kDNA loss and decreased cell proliferation. Knockdown of TbLAP1 by RNAi leads to accumulation of 2K2N cells. Recombinant Ectopic expression of HA-tagged TbLAP1 in procyclic Trypanosoma brucei induces a significant growth defect, which is manifested by the accumulation of 0K1N cells and a defect in mt membrane potential. RNAi-mediated downregulation of TbLAP1 results in a growth defect Trypanosoma brucei brucei
physiological function the leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei. TbLAP1 is a mt protein that dynamically associates with kDNA during the cell cycle. The protein localizes to kDNA and the umbilicus, the prominent proteinaceous structure which in the mammalian parasite Trypanosoma brucei connecting progeny kDNAs at late stages of segregation, indicating a role of TbLAP1 in resolving kDNA replication. TbLAP1 is required for correct segregation of kinetoplast DNA and the separation of daughter kinetoplast DNA, overview Trypanosoma brucei brucei