Cloned (Comment) | Organism |
---|---|
overexpressed in Aspergillus oryzae RIB40 | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bacitracin | 87.7% activity at 0.02 mM | Aspergillus oryzae | |
bestatin | 28.3% activity at 0.02 mM | Aspergillus oryzae | |
DTT | 3.6% activity at 1 mM | Aspergillus oryzae | |
EDTA | almost completely inhibited at 1 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.244 | - |
L-leucine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae | |
0.782 | - |
L-phenylalanine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 122% activity increase at 1 mM | Aspergillus oryzae | |
Zn2+ | 258% activity increase at 1 mM | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE, mature enzyme has the first 79 N-terminal amino acid residues removed (N-terminal sequencing) | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae RIB 40 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
from culture supernatant, ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
Leu-Leu-Leu, pH 8.5, 50°C | Aspergillus oryzae |
142 | - |
L-leucine-4-nitroanilide, pH 8.5, 50°C, 50% activity in the presence of 1 M NaCl | Aspergillus oryzae |
202 | - |
L-leucine-4-nitroanilide, pH 8.5, 60°C | Aspergillus oryzae |
221 | - |
Leu-Arg-Pro-Gly, pH 8.5, 50°C | Aspergillus oryzae |
262.3 | - |
Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu, pH 8.5, 50°C | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine-4-nitroanilide + H2O | 16.5% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae | L-arginine + 4-nitroaniline | - |
? | |
L-leucine-4-nitroanilide + H2O | - |
Aspergillus oryzae | L-leucine + 4-nitroaniline | - |
? | |
L-leucine-4-nitroanilide + H2O | - |
Aspergillus oryzae RIB 40 | L-leucine + 4-nitroaniline | - |
? | |
L-lysine-4-nitroanilide + H2O | 24.5% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae | L-lysine + 4-nitroaniline | - |
? | |
L-lysine-4-nitroanilide + H2O | 24.5% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae RIB 40 | L-lysine + 4-nitroaniline | - |
? | |
L-methionine-4-nitroanilide + H2O | 29.6% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae | L-methionine + 4-nitroaniline | - |
? | |
L-methionine-4-nitroanilide + H2O | 29.6% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae RIB 40 | L-methionine + 4-nitroaniline | - |
? | |
L-phenylalanine-4-nitroanilide + H2O | 38.8% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae | L-phenylalanine + 4-nitroaniline | - |
? | |
L-phenylalanine-4-nitroanilide + H2O | 38.8% activity in comparison to L-leucine-4-nitroanilide | Aspergillus oryzae RIB 40 | L-phenylalanine + 4-nitroaniline | - |
? | |
Leu-Arg-Pro-Gly + H2O | - |
Aspergillus oryzae | Leu + Arg-Pro-Gly | - |
? | |
Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu + H2O | - |
Aspergillus oryzae | Leu + Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu | - |
? | |
Leu-Leu-Leu + H2O | - |
Aspergillus oryzae | Leu + Leu-Leu | - |
? | |
Leu-Leu-Leu + H2O | - |
Aspergillus oryzae RIB 40 | Leu + Leu-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE, mature enzyme has the first 79 N-terminal amino acid residues removed (N-terminal sequencing) | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to 60°C, inactive at 70°C | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
L-phenylalanine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae | |
50.9 | - |
L-leucine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | more than 80% activity and stable | Aspergillus oryzae |
Organism | Comment | Expression |
---|---|---|
Aspergillus oryzae | under alkaline condition (pH 10.0) | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.68 | - |
L-phenylalanine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae | |
209 | - |
L-leucine-4-nitroanilide | pH 8.5, 50°C | Aspergillus oryzae |