Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.5 mM, completely inhibited | Pseudomonas aeruginosa | |
1,7-phenanthroline | 0.5 mM, 93% activity | Pseudomonas aeruginosa | |
4,7-phenanthroline | 0.5 mM, 95% activity | Pseudomonas aeruginosa | |
dithiothreitol | 10 mM, completely inhibited | Pseudomonas aeruginosa | |
EDTA | 10 mM, 60% activity | Pseudomonas aeruginosa | |
EGTA | 10 mM, 48% activity | Pseudomonas aeruginosa | |
additional information | not inhibited by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, tosyl-lysine chloromethylketone, N-methylmaleimide, phosphoramidon | Pseudomonas aeruginosa | |
N-ethylmaleimide | 5 mM, 95% activity | Pseudomonas aeruginosa | |
tetraethylene pentamine | 1 mM, completely inhibited | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | AP 28 | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE, AP 28 | Pseudomonas aeruginosa |
56000 | - |
SDS-PAGE, AP 56 | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
strains Habs serotype 1, FRD2 and PAO1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.5-4 units/mg, one unit is the amount of enzyme that causes an increase in optical density at 405 nm of 1 unit/h, substrate 0.6 mM leucine-p-nitroanilide in 1 ml of 0.05 M Tris/HCl, 1 mM CaCl2, pH 8.3 | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine-p-nitroanilide + H2O | - |
Pseudomonas aeruginosa | L-leucine + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AP 28 | - |
Pseudomonas aeruginosa |
AP 56 | - |
Pseudomonas aeruginosa |
PaAP | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
conversion to a 28 kDa protein that retained enzyme activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
AP 28 | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Pseudomonas aeruginosa |