Literature summary for 3.3.2.8 extracted from

Sun, Z.; Wu, L.; Bocola, M.; Chan, H.C.S.; Lonsdale, R.; Kong, X.D.; Yuan, S.; Zhou, J.; Reetz, M.T.
Structural and computational insight into the catalytic mechanism of limonene epoxide hydrolase mutants in stereoselective transformations (2018), J. Am. Chem. Soc., 140, 310-318 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant SZ348, sitting-drop vapor diffusion method, crystallized in 2.4 M sodium/potassium phosphate, 0.1 M Tris-HCl, pH 8.5, mixing of 0.002 ml of 16 mg/ml protein solution with 0.002 ml of reservoir solution, equilibration against the reservoir solution. The complex crystals of SZ348-CYO1 are made by soaking the crystals of SZ348 in sodium/potassium phosphate, 0.1 M Tris-HCl, pH 8.5, 10 mM of cyclopentene-1,2-epoxide, and 2.5% v/v acetonitrile for 5-10 min, at 18°C, X-ray diffraction structure determination and analysis	uncultured organism

Crystallization (Comment)

Organism

mutant SZ348, sitting-drop vapor diffusion method, crystallized in 2.4 M sodium/potassium phosphate, 0.1 M Tris-HCl, pH 8.5, mixing of 0.002 ml of 16 mg/ml protein solution with 0.002 ml of reservoir solution, equilibration against the reservoir solution. The complex crystals of SZ348-CYO1 are made by soaking the crystals of SZ348 in sodium/potassium phosphate, 0.1 M Tris-HCl, pH 8.5, 10 mM of cyclopentene-1,2-epoxide, and 2.5% v/v acetonitrile for 5-10 min, at 18°C, X-ray diffraction structure determination and analysis

uncultured organism

Protein Variants

Protein Variants	Comment	Organism
I80Y/L114V/I116V	site-directed mutagenesis, mutant SZ348, no or poor activity with cyclohexene-1,2-epoxide	uncultured organism
L74F/I80F/L114V/I116V/F139V	site-directed mutagenesis, mutant SZ718	uncultured organism
L74F/M78F/I80F/L114V/I116V/F139V	site-directed mutagenesis, mutant SZ719	uncultured organism
L74F/M78F/L103V/L114V/I116V/F139V/L147V	site-directed mutagenesis, mutant SZ92	uncultured organism
L74F/M78V/I80V/L114F	site-directed mutagenesis, mutant SZ338, no activity with cyclohexene-1,2-epoxide	uncultured organism
M32V/M78V/I80V/L114FM32V/M78V/I80V/L114F	site-directed mutagenesis, mutant SZ529	uncultured organism
additional information	structural and computational insight into the catalytic mechanism of limonene epoxide hydrolase mutants in stereoselective transformations	uncultured organism

Organism

Organism	UniProt	Comment	Textmining
uncultured organism	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
cyclohexene-1,2-epoxide + H2O	-	uncultured organism	(R1,R2)-cyclohexane-1,2-diol	-	?
cyclohexene-1,2-epoxide + H2O	-	uncultured organism	(S1,S2)-cyclohexane-1,2-diol	-	?
cyclopentene-1,2-epoxide + H2O	-	uncultured organism	(R1,R2)-cyclopentane-1,2-diol	-	?
cyclopentene-1,2-epoxide + H2O	-	uncultured organism	(S1,S2)-cyclopentane-1,2-diol	-	?
additional information	stereochemistry and catalytic mechanism, overview	uncultured organism	?	-	?

Synonyms

Synonyms	Comment	Organism
LEH	-	uncultured organism
limonene epoxide hydrolase	-	uncultured organism

General Information

General Information	Comment	Organism
additional information	structural and computational insight into the catalytic mechanism of limonene epoxide hydrolase variants in stereoselective transformations, molecular dynamics simulations	uncultured organism