Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ethylene induces the enzyme expression in stem and seeds, not in leaves | Glycine max |
Cloned (Comment) | Organism |
---|---|
cDNA library construction, DNA and amino acid sequence determination of the gene encoding the enzyme, genetic organization in a small EH gene family in soybean, overexpression in Escherichia coli strain BL21(DE3) | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Glycine max | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis | Glycine max |
36000 | - |
1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis | Glycine max |
36171 | - |
1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | the soluble-type epoxide hydrolase may play a crucial role in the self-defense system of the plant | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | O49857 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from dry mature seeds by ammonium sulfate fractionation, ion exchange chromatography and chromatofocusing, the latter step allows separation from the co-purifying seed-specific PR-8 chitinase Chib2, recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 55°C for 10 min, chromatofocusing and gel filtration | Glycine max |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an epoxide + H2O = a glycol | the catalytic triad is formed by Asp126, Asp285, and His320 | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | nearly no expression in leaves | Glycine max | - |
seed | mature, dry seeds, high enzyme expression level at the very early stage of seed development 13 days after flowering, the expression decreases gradually duting seed development and is nearly ceased at the very late stage 58 days after flowering | Glycine max | - |
stem | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the soluble-type epoxide hydrolase may play a crucial role in the self-defense system of the plant | Glycine max | ? | - |
? | |
styrene oxide + H2O | - |
Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis | Glycine max |
More | the enzyme contains an N-terminal transit peptide | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
soluble-type epoxide hydrolase | - |
Glycine max |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Glycine max | isoelectric focusing | - |
5.2 |