Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for enantioselective bio-organic synthesis of chiral substances | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, functional expression of wild-type enzyme and expression of enzyme mutants in Escherichia coli strain BL21(DE3) | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
D192A | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
D192N | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
D192S | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
D348A | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
D348E | site directed mutagenesis, the mutant shows 48% of wild-type enzyme activity | Aspergillus niger |
H374K | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
H374N | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
H374S | site directed mutagenesis, inactive or nearly inactive mutant, recombinantly expressed mutant enzyme is not soluble but remains in the particulate fraction of Escherichia coli cells | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, 3.3.2.9 | Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
recombinant enzyme, gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | Q9UR30 | - |
- |
Aspergillus niger LCP521 | Q9UR30 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus niger |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an epoxide + H2O = a glycol | the catalytic triad is formed by Asp192, Asp348, and His374 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-4-nitrostyrene oxide + H2O | - |
Aspergillus niger | ? | - |
? | |
(R)-4-nitrostyrene oxide + H2O | - |
Aspergillus niger LCP521 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, EC 3.3.2.9 | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
EH | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aspergillus niger |