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Literature summary for 3.3.2.10 extracted from

  • Blee, E.; Schuber, F.
    Occurence of fatty acid epoxide hydrolases in soybean (Glycine max) (1992), Biochem. J., 282, 711-714.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.10

Inhibitors

Inhibitors Comment Organism Structure
m-chloroperbenzoic acid
-
 Glycine max
styrene oxide inhibits hydration of trans-9,10-epoxystearate Glycine max
trans-9,10-epoxystearate 1 mM, 20% inhibition of hydration of 0.025 mM cis-9,10-epoxystearate Glycine max
trans-stilbene oxide inhibits hydration of trans-9,10-epoxystearate Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 cis-9,10-epoxystearate microsomal enzyme Glycine max
0.045
-
 cis-9,10-epoxystearate soluble enzyme Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Glycine max 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Glycine max
64200
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-9,10-epoxystearate + H2O
-
 Glycine max threo-9,10-dihydroxystearate
-
 ?
trans-9,10-epoxystearate + H2O
-
 Glycine max threo-9,10-dihydroxystearate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 pH 5.5: about 30% of maximal activity, pH 9.5: about 25% of maximal activity Glycine max