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Literature summary for 3.2.2.9 extracted from

  • Park, E.Y.; Choi, W.S.; Oh, S.I.; Kim, K.N.; Shin, J.S.; Song, H.K.
    Biochemical and structural characterization of 5-methylthioadenosine nucleosidases from Arabidopsis thaliana (2009), Biochem. Biophys. Res. Commun., 381, 619-624.
    View publication on PubMed
Search for more literature for 3.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) RIL cells Arabidopsis thaliana
isozyme AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5) and 26-32% (w/v) PEG MME 2000 Arabidopsis thaliana
isozyme AtMTAN2, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modelling Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2009
-
 S-adenosyl-L-homocysteine in 100 mM sodium phosphate buffer, at pH 8.0 and 37°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Arabidopsis thaliana isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not adenine + 5-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O Arabidopsis thaliana
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isozyme AtMTAN2
-
Arabidopsis thaliana Q7XA67
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione column chromatography, HiTrap Q Sepharose column chromatography, and Superose 12 gel filtration Arabidopsis thaliana
recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificities of isozymes, both isozymes are active with S-methyl-5'-thioadenosine, EC 3.2.2.16, overview Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-homocysteine + H2O isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not Arabidopsis thaliana adenine + 5-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Arabidopsis thaliana S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?

Subunits

Subunits Comment Organism
More secondary structure of isozyme AtMTAN2, structure comparison e.g. of a flexible loop, overview. Active site structures and structure modelling, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine nucleosidase
-
 Arabidopsis thaliana
5'-methylthioadenosine nucleosidases
-
 Arabidopsis thaliana
MTAN
-
 Arabidopsis thaliana
MTAN2
-
 Arabidopsis thaliana