Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Campylobacter jejuni subsp. jejuni serotype O:2 |
Crystallization (Comment) | Organism |
---|---|
structure of apoenzyme at 1.25 A, and five enzyme complexes with transition-state analogues at 1.42 to 1.95 A resolution. Inhibitor binding induces a loop movement to create a closed catalytic site with Asp196 and Ile152 providing purine leaving group activation and Arg192 and Glu12 activating the water nucleophile | Campylobacter jejuni subsp. jejuni serotype O:2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol | transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range | Campylobacter jejuni subsp. jejuni serotype O:2 | |
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol | transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range | Campylobacter jejuni subsp. jejuni serotype O:2 | |
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(2,3-dihydropyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol | transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range | Campylobacter jejuni subsp. jejuni serotype O:2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
6-amino-6-deoxyfutalosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0071 | - |
5'-methylthioadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0075 | - |
adenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0122 | - |
S-5'-adenosyl-L-homocysteine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0214 | - |
5'-deoxyadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni subsp. jejuni serotype O:2 | Q0PC20 | - |
- |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | Q0PC20 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-deoxyadenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 | adenine + 5-deoxyribose | - |
? | |
5'-deoxyadenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | adenine + 5-deoxyribose | - |
? | |
5'-methylthioadenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 | adenine + S-methyl-5'-thioribose | - |
? | |
5'-methylthioadenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | adenine + S-methyl-5'-thioribose | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 | adenine + dehypoxanthinyl futalosine | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | adenine + dehypoxanthinyl futalosine | - |
? | |
adenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 | adenine + D-ribose | - |
? | |
adenosine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | adenine + D-ribose | - |
? | |
S-5'-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-5'-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 | adenine + S-ribosyl-L-homocysteine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
6-amino-6-deoxyfutalosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.3 | - |
adenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.55 | - |
S-5'-adenosyl-L-homocysteine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.8 | - |
5'-methylthioadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.92 | - |
5'-deoxyadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000033 | - |
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0000041 | - |
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
0.0000057 | - |
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(2,3-dihydropyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
adenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
43 | - |
5'-deoxyadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
46 | - |
S-5'-adenosyl-L-homocysteine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
78 | - |
6-amino-6-deoxyfutalosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 | |
113 | - |
5'-methylthioadenosine | pH 7.4, 25°C | Campylobacter jejuni subsp. jejuni serotype O:2 |