Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, mutants E14Q (0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350, 0.2 M NaCl), D199N (0.1 M bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M MgCl2-6H2O) and D199A (0.1 M bis-tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M Li2SO4-H2O) | Helicobacter pylori |
wild type and mutant enzymes E14Q, D199N, and D199A, sitting drop vapor diffusion method, using 4.0 M NaH2PO4/K2HPO4 pH 7.0 (wild type), 0.1 M HEPES pH 7.5, 25% PEG 3350, 0.2 M NaCl (E14Q), 0.1 M bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M MgCl2-6H2O (D199N), and 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M Li2SO4-H2O (D199A) | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
D199A | inactive | Helicobacter pylori |
D199A | active-site mutant | Helicobacter pylori |
D199N | active-site mutant | Helicobacter pylori |
D199N | the mutant shows residual activity | Helicobacter pylori |
E14Q | active-site mutant | Helicobacter pylori |
E14Q | the mutant shows residual activity | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-amino-6-deoxyfutalosine + H2O | Helicobacter pylori | - |
dehypoxanthine futalosine + adenine | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Helicobacter pylori | - |
adenine + dehypoxanthinyl futalosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | O24915 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Helicobacter pylori |
HisTrap column chromatography and Superdex 75 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-amino-6-deoxyfutalosine + H2O | - |
Helicobacter pylori | dehypoxanthine futalosine + adenine | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Helicobacter pylori | adenine + dehypoxanthinyl futalosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
methylthioadenosine nucleosidase | - |
Helicobacter pylori |
MqnB | - |
Helicobacter pylori |
MTAN | - |
Helicobacter pylori |