Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-Gold cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with a 28-base pair DNA containing a guanine:5-hydroxymethyluracil mismatch, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxylcytosine-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-carboxylcytosine + double-stranded DNA with abasic site | - |
? | |
5-formylcytosine-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-formylcytosine + double-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
thymine-mismatched double-stranded DNA + H2O | Homo sapiens | - |
thymine + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13569 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxylcytosine-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-carboxylcytosine + double-stranded DNA with abasic site | - |
? | |
5-formylcytosine-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-formylcytosine + double-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
thymine-mismatched double-stranded DNA + H2O | - |
Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TDG | - |
Homo sapiens |
thymine DNA glycosylase | - |
Homo sapiens |