Cloned (Comment) | Organism |
---|---|
expression of His6-tagged ME1 in Escherichia coli strain BL21(DE3) for which ME1 is nontoxic | Mirabilis expansa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
28S rRNA + H2O | Mirabilis expansa | inactivation of protein translation in targeted cells, e.g. bacterial cells, where the toxin is transported into the cytosol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mirabilis expansa | Q8GV51 | andean root crop, type I ribosome-inactivating protein ME1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged ME1 from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, using a polyclonal anti-ME1 antibody resin, and anion exchange chromatography | Mirabilis expansa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | storage root | Mirabilis expansa | - |
storage tissue | - |
Mirabilis expansa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
28S rRNA + H2O | inactivation of protein translation in targeted cells, e.g. bacterial cells, where the toxin is transported into the cytosol | Mirabilis expansa | ? | - |
? | |
28S rRNA + H2O | Saccharomyces cerevisiae ribosomes, depurination of rRNA | Mirabilis expansa | ? | - |
? | |
additional information | no activity with Escherichia coli ribosomes by native and recombinant enzyme | Mirabilis expansa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling of ME1 | Mirabilis expansa |
Synonyms | Comment | Organism |
---|---|---|
ME1 | - |
Mirabilis expansa |
type I ribosome-inactivating protein | - |
Mirabilis expansa |
type I RIP | - |
Mirabilis expansa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mirabilis expansa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mirabilis expansa |