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Literature summary for 3.2.1.B47 extracted from

  • Furukawa, S.; Fujikawa, T.; Koga, D.; Ide, A.
    Purification and some properties of exo-type fucoidanases from Vibrio sp. N-5 (1992), Biosci. Biotechnol. Biochem., 56, 1829-1834 .
No PubMed abstract available
Search for more literature for 3.2.1.B47

Activating Compound

Activating Compound Comment Organism Structure
Co2+ 2 mM, slight activation Vibrio sp.
Mn2+ 2 mM, slight activation Vibrio sp.
Sn2+ 2 mM, slight activation Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 2 mM, 2-10% inhibition Vibrio sp.
FeCl3 2 mM, 70-80% loss of activity Vibrio sp.
HgCl2 2 mM, about 95% loss of activity Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 fucoidanase E-1, SDS-PAGE Vibrio sp.
67500
-
 fucoidanase E-3, gel filtration Vibrio sp.
68000
-
 fucoidanase E-2, SDS-PAGE Vibrio sp.
68000
-
 fucoidanase E-3, SDS-PAGE Vibrio sp.
68500
-
 fucoidanase E-2, gel filtration Vibrio sp.
158000
-
 fucoidanase E-1, gel filtration Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-
Vibrio sp. N-5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio sp.

Storage Stability

Storage Stability Organism
0°C, pH 7.5, 50 mM Tris-HCl buffer, stable for at least 6 months Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fucoidan + H2O the enzyme hydrolyzes gagome- and mozuku-fucoidans to the same extent, but not futomozuku-fucoidan Vibrio sp. ?
-
 ?
fucoidan + H2O the enzyme hydrolyzes gagome- and mozuku-fucoidans to the same extent, but not futomozuku-fucoidan Vibrio sp. N-5 ?
-
 ?
additional information no hydrolysis of 4-nitrophenyl-alpha-L-fucopyranoside Vibrio sp. ?
-
 ?
additional information no hydrolysis of 4-nitrophenyl-alpha-L-fucopyranoside Vibrio sp. N-5 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, fucoidanase E-2, SDS-PAGE Vibrio sp.
monomer 1 * 68000, fucoidanase E-3, SDS-PAGE Vibrio sp.
tetramer 4 * 39500, fucoidanase E-1, SDS-PAGE Vibrio sp.

Synonyms

Synonyms Comment Organism
exo-type fucoidanase
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6
-
 fucoidanase E-1 Vibrio sp.
6
-
 fucoidanase E-2 Vibrio sp.
7.5
-
 fucoidanase E-3 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 stable Vibrio sp.

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio sp. fucoidanase E-2, chromatofocusing
-
 5.75
Vibrio sp. fucoidanase E-1, chromatofocusing
-
 5.8
Vibrio sp. fucoidanase E-3, chromatofocusing
-
 7.65