Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, about 1.1fold activation | Talaromyces pinophilus | |
EDTA | 10 mM, about 1.1fold activation | Talaromyces pinophilus | |
glutathione | 10 mM, about 1.2fold activation | Talaromyces pinophilus |
Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is potentially a powerful biocatalyst for environmentally friendly and cost-effective production of glycyrrhetinic acid 3-O-mono-beta-D-glucuronide | Talaromyces pinophilus |
Cloned (Comment) | Organism |
---|---|
heterologous expression in Pichia pastoris | Talaromyces pinophilus |
Protein Variants | Comment | Organism |
---|---|---|
C331A | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at less than 5% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
C331D | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 25% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
C331H | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 30% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
C331K | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 25% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
C331L | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 65% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
C331S | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 30% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365A | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 40% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365C | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 50% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365D | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 20% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365K | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 60% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365L | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 70% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
H365S | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 50% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330A | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 5% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330C | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 40% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330D | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 45% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330F | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 40% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330N | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 55% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y330R | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 55% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372A | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at less than 5% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372C | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 60% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372D | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 20% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372K | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 20% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372L | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 70% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Y372S | the mutant enzyme catalyzes the hydrolysis of glycyrrhizin at about 55% of the activity compared to the wild-type enzyme | Talaromyces pinophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 10 mM, more than 90% loss of activity | Talaromyces pinophilus | |
Ca2+ | 10 mM, about 35% loss of activity | Talaromyces pinophilus | |
Cu2+ | 10 mM, more than 90% loss of activity | Talaromyces pinophilus | |
Fe2+ | 10 mM, more than 90% loss of activity | Talaromyces pinophilus | |
Mn2+ | 10 mM, about 40% loss of activity | Talaromyces pinophilus | |
Ni2+ | 10 mM, about 35% loss of activity | Talaromyces pinophilus | |
SDS | 5 mM, about 70% loss of activity | Talaromyces pinophilus | |
Triton X-100 | 5 mM, about 40% loss of activity | Talaromyces pinophilus | |
Zn2+ | 10 mM, about 60% loss of activity | Talaromyces pinophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
glycyrrhizin | pH 4.5, 45°C | Talaromyces pinophilus | |
0.76 | - |
quercetin 3-O-beta-D-glucuronide | pH 4.5, 45°C | Talaromyces pinophilus | |
6.88 | - |
Baicalin | pH 4.5, 45°C | Talaromyces pinophilus | |
25.56 | - |
4-nitrophenyl beta-D-glucuronopyranoside | pH 4.5, 45°C | Talaromyces pinophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Talaromyces pinophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47200 | - |
calculated from sequence | Talaromyces pinophilus |
85000 | - |
SDS-PAGE | Talaromyces pinophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces pinophilus | - |
- |
- |
Talaromyces pinophilus Li-93 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | highly N-glycosylated protein | Talaromyces pinophilus |
Purification (Comment) | Organism |
---|---|
- |
Talaromyces pinophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.97 | - |
pH 4.5, 45°C | Talaromyces pinophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucuronopyranoside + H2O | at 5.8% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus | 4-nitrophenol + D-glucuronate | - |
? | |
4-nitrophenyl beta-D-glucuronopyranoside + H2O | at 5.8% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus Li-93 | 4-nitrophenol + D-glucuronate | - |
? | |
baicalin + H2O | at 13.2% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus | 5,6,7-trihydroxyflavone + D-glucuronate | - |
? | |
baicalin + H2O | at 13.2% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus Li-93 | 5,6,7-trihydroxyflavone + D-glucuronate | - |
? | |
glycyrrhizin + H2O | the enzyme recognizes and hydrolyzed the distal glucuronic bond of glycyrrhizin but can not cleave the glucuronic bond in glycyrrhetinic acid 3-O-mono-beta-D-glucuronide | Talaromyces pinophilus | glycyrrhetinate 3-O-beta-D-glucuronide + D-glucuronate | - |
? | |
glycyrrhizin + H2O | the enzyme recognizes and hydrolyzed the distal glucuronic bond of glycyrrhizin but can not cleave the glucuronic bond in glycyrrhetinic acid 3-O-mono-beta-D-glucuronide | Talaromyces pinophilus Li-93 | glycyrrhetinate 3-O-beta-D-glucuronide + D-glucuronate | - |
? | |
quercetin 3-O-beta-D-glucuronide + H2O | at 51.2% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus | quercetin + D-glucuronate | - |
? | |
quercetin 3-O-beta-D-glucuronide + H2O | at 51.2% as compared to hydrolysis of glycyrrhizin | Talaromyces pinophilus Li-93 | quercetin + D-glucuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, SDS-PAGE, N-glycosylated protein | Talaromyces pinophilus |
Synonyms | Comment | Organism |
---|---|---|
glycyrrhizin glucuronohydrolase (glycyrrhetinic acid 3-O-mono-beta-D-glucuronide-forming) | - |
Talaromyces pinophilus |
TCE0_044r16781 | - |
Talaromyces pinophilus |
TpGUS79A | - |
Talaromyces pinophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Talaromyces pinophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 35°C: about 50% of maximal activity, 60°C: about 40% of maximal activity | Talaromyces pinophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, stable | Talaromyces pinophilus |
60 | - |
30 min, the enzyme loses most of its activity | Talaromyces pinophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-nitrophenyl beta-D-glucuronopyranoside | pH 4.5, 45°C | Talaromyces pinophilus | |
2.9 | - |
Baicalin | pH 4.5, 45°C | Talaromyces pinophilus | |
2.9 | - |
quercetin 3-O-beta-D-glucuronide | pH 4.5, 45°C | Talaromyces pinophilus | |
6 | - |
glycyrrhizin | pH 4.5, 45°C | Talaromyces pinophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Talaromyces pinophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | pH 4.0: about 30% of maximal activity, pH 5.5: about 30% of maximal activity | Talaromyces pinophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 7.5 | the enzyme is stable in the pH range of 2.0 to 7.5 at 4°C for 12 h | Talaromyces pinophilus |
Organism | Comment | Expression |
---|---|---|
Talaromyces pinophilus | induced by glycyrrhizin | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
4-nitrophenyl beta-D-glucuronopyranoside | pH 4.5, 45°C | Talaromyces pinophilus | |
0.29 | - |
Baicalin | pH 4.5, 45°C | Talaromyces pinophilus | |
3.83 | - |
quercetin 3-O-beta-D-glucuronide | pH 4.5, 45°C | Talaromyces pinophilus | |
11.14 | - |
glycyrrhizin | pH 4.5, 45°C | Talaromyces pinophilus |