Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
n-dodecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.05 | - |
n-undecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.08 | - |
n-decyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.08 | - |
n-nonyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.1 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.14 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.2 | - |
n-octyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.35 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.54 | - |
n-hexyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
1.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
1.96 | - |
Salicin | pH 6.0, 90°C | Pyrococcus horikoshii | |
2.02 | - |
n-amyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
40.74 | - |
methyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
138.23 | - |
beta-D-Glc-(1->3)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii | |
1698.18 | - |
beta-D-Glc-(1->4)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pyrococcus horikoshii | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58104 | - |
- |
Pyrococcus horikoshii OT-3 | O58104 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Renatured (Comment) | Organism |
---|---|
denaturation with 8 M urea and the renaturation by direct dilution with buffer | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii OT-3 | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii OT-3 | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + D-mannose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside | Pyrococcus horikoshii OT-3 | 4-nitrophenol + D-xylose | - |
? | |
beta-D-Glc-(1->3)-beta-D-Glc + H2O | i.e. laminaribiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) | Pyrococcus horikoshii | beta-D-glucose | - |
? | |
beta-D-Glc-(1->4)-beta-D-Glc + H2O | i.e. cellobiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) | Pyrococcus horikoshii | beta-D-glucose | - |
? | |
beta-D-Glc-(1->6)-beta-D-Glc + H2O | beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6) | Pyrococcus horikoshii | beta-D-glucose | - |
? | |
methyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii | methanol + beta-D-glucose | - |
? | |
methyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii OT-3 | methanol + beta-D-glucose | - |
? | |
additional information | the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus | Pyrococcus horikoshii | ? | - |
? | |
additional information | the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus | Pyrococcus horikoshii OT-3 | ? | - |
? | |
n-amyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii | 1-pentanol + beta-D-glucose | - |
? | |
n-decyl beta-D-glucopyranoside + H2O | the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains | Pyrococcus horikoshii | 1-decanol + beta-D-glucose | - |
? | |
n-dodecyl beta-D-glucopyranoside + H2O | the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains | Pyrococcus horikoshii | 1-dodecanol + beta-D-glucose | - |
? | |
n-hexyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii | 1-hexanol + beta-D-glucose | - |
? | |
n-nonyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii | 1-nonanol + beta-D-glucose | - |
? | |
n-octyl beta-D-glucopyranoside + H2O | - |
Pyrococcus horikoshii | 1-octanol + beta-D-glucose | - |
? | |
n-undecyl beta-D-glucopyranoside + H2O | the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains | Pyrococcus horikoshii | 1-undecanol + beta-D-glucose | - |
? | |
salicin + H2O | - |
Pyrococcus horikoshii | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BGPh | - |
Pyrococcus horikoshii |
PH0366 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
pH 6.0, half-life: 15 h | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
3 | 6 | n-dodecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
3 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
31 | - |
n-amyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
33 | - |
n-hexyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
34 | - |
n-octyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
35 | - |
methyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
37 | - |
n-decyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
39 | - |
n-nonyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
43 | - |
n-undecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
44 | - |
Salicin | pH 6.0, 90°C | Pyrococcus horikoshii | |
79 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
123 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
184 | - |
beta-D-Glc-(1->3)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii | |
194 | - |
beta-D-Glc-(1->4)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
beta-D-Glc-(1->4)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii | |
0.85 | - |
methyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
1.33 | - |
beta-D-Glc-(1->3)-beta-D-Glc | pH 6.0, 90°C | Pyrococcus horikoshii | |
14.6 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
15.11 | - |
n-amyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
22.2 | - |
Salicin | pH 6.0, 90°C | Pyrococcus horikoshii | |
31.83 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
60.28 | - |
n-hexyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
94.34 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
170.7 | - |
n-octyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
225.67 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
469.62 | - |
n-decyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
471.57 | - |
n-nonyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
944.37 | - |
n-undecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii | |
1152.9 | - |
n-dodecyl beta-D-glucopyranoside | pH 6.0, 90°C | Pyrococcus horikoshii |