Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 49000, small angle X-ray scattering | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A5IKD4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating affinity column chromatography and Superdex 75 gel filtration | Thermotoga petrophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
152.1 | - |
purified protein, using L-arabinan as substrate, at pH 6.0 and 70°C | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1->5)-L-alphaarabinan + H2O | - |
Thermotoga petrophila | (1->5)-L-arabinooligosaccharides | - |
? | |
additional information | the enzyme acts on branched arabinan (from sugar beet), but more slowly when compared to linear or debranched arabinan | Thermotoga petrophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, small angle X-ray scattering | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
ABNA | - |
Thermotoga petrophila |
endo-1,5-alpha-L-arabinanase | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | 80 | the enzyme shows relative activity ranging from 77% to 78% at temperatures of 62°C and 80°C, respectively | Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 95 | the enzyme is fully heat-stable (up to 10 h) and retains activity at temperatures up to 90°C. The enzyme loses its activity only after 90 min when incubated at 95°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | the enzyme retains more than 75% activity over a pH range from 4.0 to 7.0 | Thermotoga petrophila |