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Literature summary for 3.2.1.91 extracted from

  • Wahab, A.F.F.A.; Abdul Karim, N.A.; Ling, J.G.; Hasan, N.S.; Yong, H.Y.; Bharudin, I.; Kamaruddin, S.; Abu Bakar, F.D.; Murad, A.M.A.
    Functional characterisation of cellobiohydrolase I (Cbh1) from Trichoderma virens UKM1 expressed in Aspergillus niger (2019), Protein Expr. Purif., 154, 52-61 .
    View publication on PubMed
Search for more literature for 3.2.1.91

Activating Compound

Activating Compound Comment Organism Structure
Tween 80 110.12% activity at 1% (v/v) Trichoderma virens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus niger strain PY11 Trichoderma virens

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibitor Trichoderma virens
Co2+ 75.74% residual activity at 5 mM Trichoderma virens
Cu2+ 67.43% residual activity at 5 mM Trichoderma virens
D-glucose non-competitive inhibitor Trichoderma virens
EDTA 98.9% residual activity at 5 mM Trichoderma virens
K+ 90.66% residual activity at 5 mM Trichoderma virens
Mg2+ 68.12% residual activity at 5 mM Trichoderma virens
Na+ 96.61% residual activity at 5 mM Trichoderma virens
Ni2+ 70.55% residual activity at 5 mM Trichoderma virens
SDS 25.31% residual activity at 1% (w/v) Trichoderma virens
Zn2+ 91.5% residual activity at 5 mM Trichoderma virens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.88
-
 4-nitrophenyl beta-D-cellobioside at pH 4.0 and 60°C Trichoderma virens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 107.14% activity at 5 mM Trichoderma virens
Mn2+ 113.88% activity at 5 mM Trichoderma virens

Organism

Organism UniProt Comment Textmining
Trichoderma virens
-
-
-
Trichoderma virens UKM1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has two potential N-glycosylation sites at Asn-283 and Asn-397 Trichoderma virens

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and anion exchange column chromatography Trichoderma virens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.51
-
 crude enzyme, at pH 4.0 and 60°C Trichoderma virens
4.195
-
 after 8.22fold purification, at pH 4.0 and 60°C Trichoderma virens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Trichoderma virens 4-methylumbelliferone + cellobiose
-
 ?
4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Trichoderma virens UKM1 4-methylumbelliferone + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma virens 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma virens UKM1 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Trichoderma virens 4-nitrophenol + beta-D-lactopyranose
-
 ?
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Trichoderma virens UKM1 4-nitrophenol + beta-D-lactopyranose
-
 ?
avicel + H2O
-
 Trichoderma virens ?
-
 ?
avicel + H2O
-
 Trichoderma virens UKM1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, recombinant enzyme, SDS-PAGE Trichoderma virens
? x * 53280, calculated from amino acid sequence Trichoderma virens

Synonyms

Synonyms Comment Organism
CBH1
-
 Trichoderma virens
cellobiohydrolase I
-
 Trichoderma virens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Trichoderma virens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is stable for 1 h up to 60°C Trichoderma virens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Trichoderma virens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 the enzyme is stable for 1 h at pH 3.0 to 6.0 in acetate buffer Trichoderma virens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.65
-
 cellobiose at pH 4.0 and 60°C Trichoderma virens
24.61
-
 D-glucose at pH 4.0 and 60°C Trichoderma virens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
56800
-
 4-nitrophenyl beta-D-cellobioside at pH 4.0 and 60°C Trichoderma virens