BRENDA - Enzyme Database
show all sequences of 3.2.1.86

Characterization of a novel metagenome-derived 6-phospho-beta-glucosidase from black liquor sediment

Yang, C.; Niu, Y.; Li, C.; Zhu, D.; Wang, W.; Liu, X.; Cheng, B.; Ma, C.; Xu, P.; Appl. Environ. Microbiol. 79, 2121-2127 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned from metagenome, library construction, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain Rosetta (DE3)
uncultured organism
Engineering
Amino acid exchange
Commentary
Organism
E179A
site-directed mutagenesis, inactive mutant
uncultured organism
E372A
site-directed mutagenesis, inactive mutant
uncultured organism
K435L
site-directed mutagenesis, inactive mutant
uncultured organism
S427A
site-directed mutagenesis, the mutant shows slightly decreased activity but substrate affinities compared to the wild-type enzyme
uncultured organism
S427C
site-directed mutagenesis, the mutant shows increased activity but substrate affinities compared to the wild-type enzyme
uncultured organism
S427D
site-directed mutagenesis, almost inactive mutant
uncultured organism
S427E
site-directed mutagenesis, almost inactive mutant
uncultured organism
W420A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
W420F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
Y311F
site-directed mutagenesis, inactive mutant
uncultured organism
Y437F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
uncultured organism
Fe2+
-
uncultured organism
Fe3+
-
uncultured organism
Ni2+
-
uncultured organism
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.8
-
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.0, 37°C
uncultured organism
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
stabilizes the enzyme activity
uncultured organism
Mg2+
stabilizes the enzyme activity
uncultured organism
Mn2+
stabilizes the enzyme activity
uncultured organism
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
uncultured organism
L7Y2Z6
collected from pulp wastewater from a pulp mill in China
-
Purification (Commentary)
Commentary
Organism
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nickel afinity and anion exchange and again nickel affinity chromatography, followed by ultrafiltration
uncultured organism
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
-
731178
uncultured organism
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
additional information
the enzyme shows no beta-glucosidase activity, EC 3.2.1.21
731178
uncultured organism
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
uncultured organism
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
uncultured organism
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned from metagenome, library construction, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain Rosetta (DE3)
uncultured organism
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E179A
site-directed mutagenesis, inactive mutant
uncultured organism
E372A
site-directed mutagenesis, inactive mutant
uncultured organism
K435L
site-directed mutagenesis, inactive mutant
uncultured organism
S427A
site-directed mutagenesis, the mutant shows slightly decreased activity but substrate affinities compared to the wild-type enzyme
uncultured organism
S427C
site-directed mutagenesis, the mutant shows increased activity but substrate affinities compared to the wild-type enzyme
uncultured organism
S427D
site-directed mutagenesis, almost inactive mutant
uncultured organism
S427E
site-directed mutagenesis, almost inactive mutant
uncultured organism
W420A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
W420F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
Y311F
site-directed mutagenesis, inactive mutant
uncultured organism
Y437F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
uncultured organism
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
uncultured organism
Fe2+
-
uncultured organism
Fe3+
-
uncultured organism
Ni2+
-
uncultured organism
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.8
-
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.0, 37°C
uncultured organism
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
stabilizes the enzyme activity
uncultured organism
Mg2+
stabilizes the enzyme activity
uncultured organism
Mn2+
stabilizes the enzyme activity
uncultured organism
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nickel afinity and anion exchange and again nickel affinity chromatography, followed by ultrafiltration
uncultured organism
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
-
731178
uncultured organism
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
additional information
the enzyme shows no beta-glucosidase activity, EC 3.2.1.21
731178
uncultured organism
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
uncultured organism
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
uncultured organism
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
uncultured organism
additional information
conserved residues S427, Lys435, and Tyr437 act as gatekeepers in a phosphate-binding loop and play important roles in phosphate recognition, homology structure modelling, overview
uncultured organism
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
uncultured organism
additional information
conserved residues S427, Lys435, and Tyr437 act as gatekeepers in a phosphate-binding loop and play important roles in phosphate recognition, homology structure modelling, overview
uncultured organism
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T-1
FEBS J.
284
3931-3953
2017
-
-
-
1
3
-
-
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1
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-
3
-
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1
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-
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2
1
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1
3
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1
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1
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2
1
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-
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-
-
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1
-
-
1
-
-
751192
Yang
-
Coloring and bioinformatic an ...
Oenococcus oeni
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17
197-205
2017
-
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
750546
Kwan
Chemoenzymatic synthesis of 6 ...
Streptococcus pyogenes, Streptococcus pyogenes M1
FEBS Lett.
590
461-468
2016
-
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1
1
-
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1
1
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-
-
-
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2
-
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1
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2
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1
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1
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1
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1
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1
1
1
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1
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-
2
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
750733
Terra
Pneumococcal 6-phospho-beta-g ...
Streptococcus pneumoniae
Infect. Immun.
84
286-292
2016
-
-
1
-
-
-
-
1
1
-
-
2
-
4
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
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1
1
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2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750963
Zangoui
Evolution of aromatic beta-gl ...
Escherichia coli
J. Bacteriol.
197
710-716
2015
-
-
-
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1
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1
-
1
-
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-
-
4
-
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1
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1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731055
Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
Acta Crystallogr. Sect. D
69
16-23
2013
-
-
1
1
-
1
1
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
1
1
-
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1
1
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1
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1
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1
-
1
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2
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-
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-
1
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-
-
-
4
1
1
-
-
1
1
-
-
-
-
3
3
-
1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
Appl. Environ. Microbiol.
79
2121-2127
2013
-
-
1
-
11
-
4
1
-
3
-
-
-
1
-
-
1
-
-
-
-
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2
-
1
-
-
-
1
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-
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1
-
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11
-
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4
-
1
-
3
-
-
-
-
-
1
-
-
-
-
2
-
1
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-
-
1
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-
2
2
-
-
-
732101
Yu
Structural insights into the s ...
Streptococcus pneumoniae
J. Biol. Chem.
288
14949-14958
2013
-
-
1
1
13
-
-
6
-
-
-
1
-
4
-
-
1
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
-
-
-
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1
-
1
13
-
-
-
-
6
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
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1
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-
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2
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1
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6
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1
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8
1
2
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2
2
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2
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-
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2
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1
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2
-
-
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-
8
1
2
-
-
2
2
-
-
-
-
1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
J. Mol. Catal. B
69
27-34
2011
-
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1
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-
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-
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1
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1
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1
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2
1
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1
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1
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1
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1
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1
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2
1
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1
-
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1
-
-
-
-
-
-
-
-
-
705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
-
1
-
3
-
-
-
1
1
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4
-
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1
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2
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1
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1
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1
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1
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3
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1
1
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1
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2
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1
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1
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678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
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31
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2
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2
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-
-
-
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-
-
-
681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
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1
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1
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1
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Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
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2006
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11
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11
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1
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1
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11
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666041
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Thermotoga maritima
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2
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2
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666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
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-
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1
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2
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5
1
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2
1
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4
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1
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1
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3
1
1
1
1
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1
1
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1
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2
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5
-
1
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2
1
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1
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1
-
3
1
1
1
1
-
1
1
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-
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-
-
-
-
-
655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
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3
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1
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2
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1
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1
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1
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3
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1
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2
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1
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656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
-
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1
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1
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4
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1
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4
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-
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-
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-
136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
-
-
-
-
-
-
-
7
-
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2
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
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-
-
-
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-
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-
-
-
-
-
-
-
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7
-
-
2
-
-
-
-
-
-
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6
1
1
-
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-
-
-
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-
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136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
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1
9
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3
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3
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1
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1
11
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1
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1
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1
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9
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3
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1
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1
11
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1
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1
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136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
J. Bacteriol.
175
5168-5175
1993
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2
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1
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1
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136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
J. Bacteriol.
169
2579-2590
1987
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5
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2
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2
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136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
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7
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1
1
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1
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1
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1
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8
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1
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7
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1
1
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1
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1
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8
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1
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136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
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10
9
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4
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1
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1
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1
2
6
1
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2
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2
1
1
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10
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9
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4
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1
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1
2
6
1
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2
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2
1
1
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136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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1
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3
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3
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136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
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7
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1
1
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1
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1
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1
1
8
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1
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7
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1
1
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1
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1
1
8
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1
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