BRENDA - Enzyme Database show
show all sequences of 3.2.1.86

The beta-glucoside system of Escherichia coli. IV. Purification and properties of phospho-beta-glucosidases A and B

Wilson, G.; Fox, C.F.; J. Biol. Chem. 249, 5586-5598 (1974)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-AMP
-
Escherichia coli
3',5'-AMP
-
Escherichia coli
3'-AMP
-
Escherichia coli
AMP
-
Escherichia coli
ATP
-
Escherichia coli
CMP
-
Escherichia coli
DL-alpha-glycerol-phosphate
-
Escherichia coli
fructose-1,6-diphosphate
-
Escherichia coli
NADH
-
Escherichia coli
phosphoenolpyruvate
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
p-nitrothiophenyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.18
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
isoenzyme B
Escherichia coli
0.19
-
methyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.25
-
o-nitrophenyl beta-D-galactoside 6-phosphate
isoenzyme B
Escherichia coli
0.28
-
methyl beta-D-glucoside 6-phosphate
isoenzyme A
Escherichia coli
0.33
-
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate
isoenzyme A
Escherichia coli
0.46
-
phenyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.51
-
Salicin 6-phosphate
isoenzyme B
Escherichia coli
0.6
-
phenyl beta-D-glucoside 6-phosphate
isoenzyme A
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
isoenzyme B, SDS-PAGE
Escherichia coli
68000
-
isoenzyme A, SDS-PAGE
Escherichia coli
132000
-
isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement
Escherichia coli
142000
-
isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
K-12
-
Purification (Commentary)
Commentary
Organism
63 fold
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
isoenzyme A, 4°C, 1 year, sterile starting buffer, 0% loss in activity
Escherichia coli
isoenzyme B, -20°C, 2 months, sterile starting buffer, 50% loss of activity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl beta-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
?
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
136924
Escherichia coli
o-nitrophenol + D-galactose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
isoenzyme B
136924
Escherichia coli
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
?
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
phenol + glucose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
isoenzyme B
136924
Escherichia coli
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
28
isoenzyme B
Escherichia coli
25
45
isoenzyme A
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
isoenzyme A, p-nitrophenyl beta-D-glucospyranoside
Escherichia coli
7
9
isoenzyme B, p-nitrophenyl beta-D-glucospyranoside
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
10
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
10
for more than 1 h
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-AMP
-
Escherichia coli
3',5'-AMP
-
Escherichia coli
3'-AMP
-
Escherichia coli
AMP
-
Escherichia coli
ATP
-
Escherichia coli
CMP
-
Escherichia coli
DL-alpha-glycerol-phosphate
-
Escherichia coli
fructose-1,6-diphosphate
-
Escherichia coli
NADH
-
Escherichia coli
phosphoenolpyruvate
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
p-nitrothiophenyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.18
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
isoenzyme B
Escherichia coli
0.19
-
methyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.25
-
o-nitrophenyl beta-D-galactoside 6-phosphate
isoenzyme B
Escherichia coli
0.28
-
methyl beta-D-glucoside 6-phosphate
isoenzyme A
Escherichia coli
0.33
-
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate
isoenzyme A
Escherichia coli
0.46
-
phenyl beta-D-glucoside 6-phosphate
isoenzyme B
Escherichia coli
0.51
-
Salicin 6-phosphate
isoenzyme B
Escherichia coli
0.6
-
phenyl beta-D-glucoside 6-phosphate
isoenzyme A
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
isoenzyme B, SDS-PAGE
Escherichia coli
68000
-
isoenzyme A, SDS-PAGE
Escherichia coli
132000
-
isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement
Escherichia coli
142000
-
isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
63 fold
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
isoenzyme A, 4°C, 1 year, sterile starting buffer, 0% loss in activity
Escherichia coli
isoenzyme B, -20°C, 2 months, sterile starting buffer, 50% loss of activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl beta-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
?
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
136924
Escherichia coli
o-nitrophenol + D-galactose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
isoenzyme B
136924
Escherichia coli
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
?
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
-
136924
Escherichia coli
phenol + glucose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
isoenzyme B
136924
Escherichia coli
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
28
isoenzyme B
Escherichia coli
25
45
isoenzyme A
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
isoenzyme A, p-nitrophenyl beta-D-glucospyranoside
Escherichia coli
7
9
isoenzyme B, p-nitrophenyl beta-D-glucospyranoside
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
10
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
10
for more than 1 h
Escherichia coli
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731055
Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
Acta Crystallogr. Sect. D
69
16-23
2013
-
-
1
1
-
1
1
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
3
3
-
1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
Appl. Environ. Microbiol.
79
2121-2127
2013
-
-
1
-
11
-
4
1
-
3
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
11
-
-
4
-
1
-
3
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732101
Yu
Structural insights into the s ...
Streptococcus pneumoniae
J. Biol. Chem.
288
14949-14958
2013
-
-
1
1
13
-
-
6
-
-
-
1
-
4
-
-
1
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
-
-
-
-
1
-
1
13
-
-
-
-
6
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
-
1
-
-
-
-
2
-
-
1
-
-
6
-
-
1
-
-
-
-
-
8
1
2
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
2
-
-
-
-
8
1
2
-
-
2
2
-
-
-
-
1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
J. Mol. Catal. B
69
27-34
2011
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
-
1
-
3
-
-
-
1
1
-
-
-
4
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
BMC Microbiol.
7
31
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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1
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
14
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
1
-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678190
Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
45
571-580
2006
-
-
-
-
-
-
-
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
-
-
-
11
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
666041
Varrot
NAD+ and Metal-ion Dependent H ...
Thermotoga maritima
J. Mol. Biol.
346
423-435
2005
-
-
-
1
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
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1
-
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-
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2
-
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-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
Res. Microbiol.
156
145-153
2005
-
-
1
-
2
-
5
1
-
2
1
-
-
4
-
-
1
-
-
-
1
-
3
1
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
2
-
-
5
-
1
-
2
1
-
-
-
-
1
-
-
1
-
3
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
J. Am. Chem. Soc.
126
8354-8355
2004
3
-
1
-
-
-
-
1
-
3
-
1
-
1
-
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-
-
-
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-
2
-
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1
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-
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3
-
1
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-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
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4
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1
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-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
-
-
-
-
-
-
-
7
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
-
-
-
-
-
-
1
9
-
-
3
-
-
3
-
-
1
-
-
-
-
1
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
3
-
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-
-
1
-
-
-
1
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
J. Bacteriol.
175
5168-5175
1993
-
-
-
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2
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1
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1
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-
-
136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
J. Bacteriol.
169
2579-2590
1987
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4
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2
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2
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136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
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7
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8
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1
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7
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1
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1
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8
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1
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136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
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10
9
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4
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6
1
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2
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2
1
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10
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9
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4
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2
6
1
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2
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2
1
1
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136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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3
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3
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136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
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7
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8
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1
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7
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1
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1
8
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1
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