Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Agarivorans sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, complete inhibition | Agarivorans sp. | |
additional information | strong resistance to Cu2+, Pb2+ and Zn2+ at 1 mM, iodoacetamide, p-chloromercuribenzoate, dithiothreitol and 2-mercaptoethanol, SDS at 30 mM | Agarivorans sp. | |
N-bromosuccinimide | 0.1 mM | Agarivorans sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, stimulates enzyme | Agarivorans sp. | |
Na+ | 0.1 M, stimulates enzyme | Agarivorans sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
SDS-PAGE | Agarivorans sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans sp. | - |
- |
- |
Agarivorans sp. JAMB-A11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m, purification by ammonium sulfate precipitation, dialysis, DEAE-Toyopearl 650M column, dialysis | Agarivorans sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m | Agarivorans sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
371 | - |
50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 7.8, containing 10 mM CaCl2, 0.1 mM NaCl, 40°C | Agarivorans sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Agarivorans sp. | agarotetraose + ? | - |
? | |
agarose + H2O | - |
Agarivorans sp. JAMB-A11 | agarotetraose + ? | - |
? | |
neoagarotetraose + H2O | - |
Agarivorans sp. | neoagarobiose | - |
? | |
neoagarotetraose + H2O | - |
Agarivorans sp. JAMB-A11 | neoagarobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RagaA11 | - |
Agarivorans sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 7.8, containing 10 mM CaCl2, 0.1 mM NaCl | Agarivorans sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
retaining 85% of original activity after 15 min in 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer containing 0.1 M NaCl and 10 mM CaCl2 at pH 7.8 | Agarivorans sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | Britton-Robinson universal buffers, MOPS and N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer | Agarivorans sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | retaining 70% of original activity | Agarivorans sp. |