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Literature summary for 3.2.1.8 extracted from

  • Zhuo, R.; Yu, H.; Qin, X.; Ni, H.; Jiang, Z.; Ma, F.; Zhang, X.
    Heterologous expression and characterization of a xylanase and xylosidase from white rot fungi and their application in synergistic hydrolysis of lignocellulose (2018), Chemosphere, 212, 24-33 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 10 mM, activity increases by 26.4% Pleurotus ostreatus
Cr3+ 10 mM, activity increases by 28.2% Pleurotus ostreatus
Triton X-100 1%, activity increases by 29.43%. 4%, activity increases by 32.21% Pleurotus ostreatus
Tween 20 1%, activity increases by 40.21%. 4%, activity increases by 66.36% Pleurotus ostreatus
Zn2+ 10 mM, activity increases by 4.0% Pleurotus ostreatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Pleurotus ostreatus

Protein Variants

Protein Variants Comment Organism
biofuel production potential application in the field of biomass pretreatment and biofuel production Pleurotus ostreatus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 10 mM, 37% loss of activity Pleurotus ostreatus
Cu2+ 10 mM, 97% loss of activity Pleurotus ostreatus
Fe3+ 10 mM, 37% loss of activity Pleurotus ostreatus
K+ 10 mM, 33% loss of activity Pleurotus ostreatus
Li+ 10 mM, 39% loss of activity Pleurotus ostreatus
Mg2+ 10 mM, 13% loss of activity Pleurotus ostreatus
Ni2+ 10 mM, 44 % loss of activity Pleurotus ostreatus
PEG 200 4%, 46% inhibition Pleurotus ostreatus
SDS 2%, 60% loss of activity Pleurotus ostreatus
Tween 80 1%, 33.29% loss of activity Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42200
-
 calculated from sequence Pleurotus ostreatus
43000
-
 SDS-PAGE Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus A0A2U9Q8K4
-
-
Pleurotus ostreatus HAUCC 162 A0A2U9Q8K4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oat spelts xylan + H2O
-
 Pleurotus ostreatus D-xylose + ?
-
 ?
oat spelts xylan + H2O
-
 Pleurotus ostreatus HAUCC 162 D-xylose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Pleurotus ostreatus
? x * 42200, calculated from sequence Pleurotus ostreatus

Synonyms

Synonyms Comment Organism
endo-1,4-xylanase
-
 Pleurotus ostreatus
Xyn162
-
 Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pleurotus ostreatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 50% of maximal activity, 70°C: about 35% of maximal activity Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pleurotus ostreatus

General Information

General Information Comment Organism
physiological function endo-xylanase and beta-xylosidase are the major enzymes for hemicellulose hydrolysis, which play a significant role in biomass conversion Pleurotus ostreatus