Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzyl bromide | 1 mM, 27% inhibition | Microcella alkaliphila | |
Cu2+ | 1 mM, 18% inhibition | Microcella alkaliphila | |
Hg2+ | 1 mM, 1% residual activiy | Microcella alkaliphila | |
additional information | not inhibitory at 1 mM: iodoacetate, N-ethylmaleimide, o-phenanthroline, EDTA, EGTA, dithiothreitol, 2-mercaptoethanol, 1-ethyl-3-dimethyl aminopropylcarbadimide, N-bromosuccinimide, phenylmethansulfonylfluoride, 4-chloromercuribenzoate, SDS, Tween 80, and Triton X-100. Not inhibitory: Ca2+, Sr2+, Co2+, Mg2+, Pb2+, Ni2+, Mn2+, Fe2+, Sn2+, Zn2+, and Fe3+ | Microcella alkaliphila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
SDS-PAGE | Microcella alkaliphila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microcella alkaliphila | A0A0U5B5Z4 | - |
- |
Microcella alkaliphila JAM-AC0309 | A0A0U5B5Z4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | 148% of the activity with oat spelt xylan | Microcella alkaliphila | xylo-oligosaccharides + ? | - |
? | |
arabinoxylan + H2O | 148% of the activity with oat spelt xylan | Microcella alkaliphila JAM-AC0309 | xylo-oligosaccharides + ? | - |
? | |
birchwood xylan + H2O | 110% of the activity with oat spelt xylan | Microcella alkaliphila | xylo-oligosaccharides + ? | - |
? | |
birchwood xylan + H2O | 110% of the activity with oat spelt xylan | Microcella alkaliphila JAM-AC0309 | xylo-oligosaccharides + ? | - |
? | |
additional information | enzyme degrades degraded xylooligosaccharides more than xylotetraose. No substrates: avicel, carboxymethyl cellulose, lichenan, soluble starch, glucomannan, galactomannan, or arabinan, 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinopyranoside. The enzyme also catalyzes transglycosylation. Xylobiose to xylohexaose are transglycolated by xylobiose units during the early degradation step, respectively | Microcella alkaliphila | ? | - |
? | |
additional information | enzyme degrades degraded xylooligosaccharides more than xylotetraose. No substrates: avicel, carboxymethyl cellulose, lichenan, soluble starch, glucomannan, galactomannan, or arabinan, 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinopyranoside. The enzyme also catalyzes transglycosylation. Xylobiose to xylohexaose are transglycolated by xylobiose units during the early degradation step, respectively | Microcella alkaliphila JAM-AC0309 | ? | - |
? | |
oat spelt xylan + H2O | - |
Microcella alkaliphila | xylo-oligosaccharides + ? | - |
? | |
oat spelt xylan + H2O | - |
Microcella alkaliphila JAM-AC0309 | xylo-oligosaccharides + ? | - |
? | |
xylopentaose + H2O | - |
Microcella alkaliphila | xylobiose + xylotriose | - |
? | |
xylopentaose + H2O | - |
Microcella alkaliphila JAM-AC0309 | xylobiose + xylotriose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MalAC0309_0409 | - |
Microcella alkaliphila |
XynE15 | - |
Microcella alkaliphila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Microcella alkaliphila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 48 h | Microcella alkaliphila |
75 | - |
30 min, stable up to 75°C | Microcella alkaliphila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Microcella alkaliphila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | more than 80% of maximum activity | Microcella alkaliphila |