Cloned (Comment) | Organism |
---|---|
gene xyn2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme without N-terminal signal in Escherichia coli | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for beta-1,4-xylan from birchwood is 0.15 mg/ml | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Trichoderma reesei | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | B2CNY5 | Xyn2 or Rut C-30; gene xyn2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Xyn2 1.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trichoderma reesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2350 | - |
purified recombinant enzyme | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
beta-1,4-xylan + H2O | from birchwood | Trichoderma reesei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xylanase2 | - |
Trichoderma reesei |
Rut C-30 | - |
Trichoderma reesei |
xylanase | - |
Trichoderma reesei |
Xyn2 | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma reesei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30% of maximal activity at 70°C | Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
over 85% activity remaining after 30 min | Trichoderma reesei |
60 | - |
over 65% activity remaining after 30 min | Trichoderma reesei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
199.7 | - |
beta-1,4-xylan | pH 5.0, 50°C, birchwood xylan | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, 40% of maximal activity at pH 3.0 and pH 8.0 | Trichoderma reesei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | purified recombinant enzyme, stable | Trichoderma reesei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma reesei | isoelectric focusing | - |
7.5 |