Cloned (Comment) | Organism |
---|---|
XynBE18 expression in Escherichia coli strain Bl21(DE3) | Paenibacillus sp. E18 |
Protein Variants | Comment | Organism |
---|---|---|
E129A/E236A | the naturally occuring enzyme mutant XynBE18 shows also beta-1,3-1,4-glucan hydrolase activity, EC 3.2.1.6. Recombinant XynBE18 shows specificity toward oat spelt xylan and birchwood xylan and barley beta-1,3-1,4-glucan and lichenin, no activity with carboxymethylcellulose or Avicel, overview | Paenibacillus sp. E18 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Paenibacillus sp. E18 | |
Cu2+ | - |
Paenibacillus sp. E18 | |
Fe3+ | - |
Paenibacillus sp. E18 | |
Hg2+ | strong inhibition | Paenibacillus sp. E18 | |
Ni2+ | - |
Paenibacillus sp. E18 | |
SDS | - |
Paenibacillus sp. E18 | |
Zn2+ | - |
Paenibacillus sp. E18 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant effect on the xylanase activity of recombinant XynBE18 by 1-10 mM Na, K, Li, Mg2, Ca2, or EDTA | Paenibacillus sp. E18 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Paenibacillus sp. E18 | natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 | xylose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. E18 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
59.6 | - |
recombinant XynBE18, substrate oat spelt xylan | Paenibacillus sp. E18 |
61.57 | - |
recombinant XynBE18, substrate birchwood xylan | Paenibacillus sp. E18 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 | Paenibacillus sp. E18 | xylose + ? | - |
? | |
beta-1,4-xylan + H2O | from birchwood and oat spelt | Paenibacillus sp. E18 | xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | XynBE18 structure-function relationship, homology modeling and molecular dynamic simulation, overview | Paenibacillus sp. E18 |
Synonyms | Comment | Organism |
---|---|---|
XynB18 | - |
Paenibacillus sp. E18 |
XynE2 | - |
Paenibacillus sp. E18 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Paenibacillus sp. E18 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | profile, overview | Paenibacillus sp. E18 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Paenibacillus sp. E18 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 12 | activity range, profile, overview | Paenibacillus sp. E18 |