Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 10 mM enhances the activity about 1.6fold | Streptomyces sp. S9 | |
additional information | Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect | Streptomyces sp. S9 |
Application | Comment | Organism |
---|---|---|
industry | favorable properties of XynAS9, such as high activity over a wide temperature range, good thermal and pH stability, and less complex hydrolysis products, make this xylanase promising for various applications in the animal feed and biofuel industries | Streptomyces sp. S9 |
Cloned (Comment) | Organism |
---|---|
into pET-22b(+) vector and expressed in Escherichia coli BL21 (DE3) | Streptomyces sp. S9 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM strongly inhibits | Streptomyces sp. S9 | |
Co2+ | 10 mM partialy inhibits | Streptomyces sp. S9 | |
Cu2+ | 10 mM partialy inhibits | Streptomyces sp. S9 | |
Hg2+ | 1 mM completely inhibits | Streptomyces sp. S9 | |
K+ | 10 mM partialy inhibits | Streptomyces sp. S9 | |
Mn2+ | 10 mM partialy inhibits | Streptomyces sp. S9 | |
additional information | Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect | Streptomyces sp. S9 | |
Pb2+ | 10 mM partialy inhibits | Streptomyces sp. S9 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 10 mM enhances the activity about 1.3fold | Streptomyces sp. S9 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, SDS-PAGE | Streptomyces sp. S9 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. S9 | B4XVN1 | - |
- |
Purification (Comment) | Organism |
---|---|
to electrophoretic homogeneity by ammonium sulfate precipitation, ion exchange chromatography, and hydrophobic interaction chromatography, 7.1fold purified, with a final activity yield of 18.1% | Streptomyces sp. S9 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.6 | - |
culture supernatant | Streptomyces sp. S9 |
453.7 | - |
7.1fold purified enzyme | Streptomyces sp. S9 |
Storage Stability | Organism |
---|---|
4°C, 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.5 | Streptomyces sp. S9 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | xylose and xylobiose are the predominant products | Streptomyces sp. S9 | D-xylose + xylobiose + ? | - |
? | |
oat spelt xylan + H2O | xylose and xylobiose are the predominant products | Streptomyces sp. S9 | D-xylose + xylobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Streptomyces sp. S9 |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Streptomyces sp. S9 |
XynAS9 | - |
Streptomyces sp. S9 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. S9 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | retains more than 65% of the maximum activity | Streptomyces sp. S9 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces sp. S9 |