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Literature summary for 3.2.1.7 extracted from
- Efficient production of inulooligosaccharides from inulin by endoinulinase from Aspergillus arachidicola (2019), Carbohydr. Polym., 208, 70-76 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Aspergillus arachidicola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | inhibits 71.7% at 1 mM | Aspergillus arachidicola |  |
| Cu2+ | inhibits 65.9% at 1 mM | Aspergillus arachidicola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, Km for inulin is 4.62 mg/ml, Vmax is 0.0232 mmol/min/mg, recombinant enzyme | Aspergillus arachidicola |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme contains a predicted signal peptide, the enzyme is secreted | Aspergillus arachidicola | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates over 20% at 1 mM | Aspergillus arachidicola | |
| Fe3+ | stimulates over 20% at 1 mM | Aspergillus arachidicola | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus arachidicola | A0A2G7G4Y0 | - |
- |
| Aspergillus arachidicola CBS 117610 | A0A2G7G4Y0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Aspergillus arachidicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | the purified recombinant enzyme efficiently produces inulooligosaccharides from inulin, with inulotriose and inulotetraose as major products. When 10, 30, 50, and 100 g/l of inulin is used as substrate, the final conversion ratio of total inulooligosaccharides reaches 88.4%, 83.5%, 80.2%, and 60.1%, respectively. Carbohydrate compositions of reaction products from various concentrations of inulin substrate, overview | Aspergillus arachidicola | inulotriose + inulotetraose | - |
? | |
| inulin + H2O | the purified recombinant enzyme efficiently produces inulooligosaccharides from inulin, with inulotriose and inulotetraose as major products. When 10, 30, 50, and 100 g/l of inulin is used as substrate, the final conversion ratio of total inulooligosaccharides reaches 88.4%, 83.5%, 80.2%, and 60.1%, respectively. Carbohydrate compositions of reaction products from various concentrations of inulin substrate, overview | Aspergillus arachidicola CBS 117610 | inulotriose + inulotetraose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus arachidicola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AARAC_000847 | locus name | Aspergillus arachidicola |
| endoinulinase | - |
Aspergillus arachidicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus arachidicola |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | over 50% of maximal activity within this range, profile overview | Aspergillus arachidicola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, 2 h, completely stable up to | Aspergillus arachidicola |
| 50 | - |
purified recombinant enzyme, 4°C, 2 h, 85% activity remaining | Aspergillus arachidicola |
| 60 | - |
purified recombinant enzyme, 4°C, 2 h, 45% activity remaining | Aspergillus arachidicola |
| 70 | - |
purified recombinant enzyme, 4°C, 2 h, inactivation | Aspergillus arachidicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus arachidicola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | over 50% of maximal activity within this range, profile overview | Aspergillus arachidicola |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
purified recombinant enzyme, 4°C, 2 h, 50% activity remaining | Aspergillus arachidicola |
| 5 | - |
purified recombinant enzyme, 4°C, 2 h, completely stable at | Aspergillus arachidicola |
| 8 | - |
purified recombinant enzyme, 4°C, 2 h, 50% activity remaining | Aspergillus arachidicola |
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