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Literature summary for 3.2.1.7 extracted from
- Expression of an endoinulinase from Aspergillus ficuum JNSP5-06 in Escherichia coli and its characterization (2012), Carbohydr. Polym., 88, 748-753.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 5 mM, 113% of initial activity | Aspergillus ficuum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aspergillus ficuum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, no residual activity | Aspergillus ficuum | |
| Al3+ | 5 mM, 17% residual activity | Aspergillus ficuum | |
| Fe2+ | 5 mM, 30% residual activity | Aspergillus ficuum | |
| Fe3+ | 5 mM, 54% residual activity | Aspergillus ficuum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
inulin | Km value is 67.4 mg/ml, pH 5.0, 60°C | Aspergillus ficuum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, 114% of initial activity | Aspergillus ficuum | |
| Zn2+ | 5 mM, 124% of initial activity | Aspergillus ficuum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Aspergillus ficuum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ficuum | - |
- |
- |
| Aspergillus ficuum JNSP5-06 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Aspergillus ficuum | ? | major products of hydrolysis are fructo-oligosaccharides with degree of polymerization from 3 to 4 | ? | |
| inulin + H2O | - |
Aspergillus ficuum JNSP5-06 | ? | major products of hydrolysis are fructo-oligosaccharides with degree of polymerization from 3 to 4 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Aspergillus ficuum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Endo I | - |
Aspergillus ficuum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus ficuum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus ficuum |
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Release 2023.1 (January 2023)
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